TY - JOUR
T1 - Bioactive flavone fisetin attenuates hypertension associated cardiac hypertrophy in H9c2 cells and in spontaneously hypertension rats
AU - Lin, Kuan Ho
AU - Shibu, Marthandam Asokan
AU - Peramaiyan, Rajendran
AU - Chen, Ya Fang
AU - Shen, Chia Yao
AU - Hsieh, You Liang
AU - Chen, Ray Jade
AU - Viswanadha, Vijaya Padma
AU - Kuo, Wei Wen
AU - Huang, Chih Yang
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Fisetin, a naturally occurring flavonoid in fruits, nuts and vegetables possess antioxidant and anti-inflammatory properties. This study shows that fisetin exerts preventive and therapeutic effects on hypertension associated cardiac abnormalities in H9c2 cells and Spontaneously Hypertensive Rats (SHRs). For In vitro analysis, H9c2 cells were challenged with AngII and Fisetin (50 µM/mL) was administered either before 2 h or after 12 h of AngII challenge. For In vivo studies, SHRs received oral fisetin (10 mg/kg, twice a week for 6 weeks) and their cardiac function was analyzed by echocardiography. Histological studies and Western blotting were performed to assess the severity and the mechanism of the underlying cardiac abnormality. Fisetin effectively suppressed calcineurin-NFATC3 involved cellular hypertrophic effects as observed in H9c2 cells and in SHR hearts. Fisetin also enhanced cardiac function and restored cardiac morphology and therefore, fisetin is a potential agent against hypertension associated hypertrophy and cardiac deterioration.
AB - Fisetin, a naturally occurring flavonoid in fruits, nuts and vegetables possess antioxidant and anti-inflammatory properties. This study shows that fisetin exerts preventive and therapeutic effects on hypertension associated cardiac abnormalities in H9c2 cells and Spontaneously Hypertensive Rats (SHRs). For In vitro analysis, H9c2 cells were challenged with AngII and Fisetin (50 µM/mL) was administered either before 2 h or after 12 h of AngII challenge. For In vivo studies, SHRs received oral fisetin (10 mg/kg, twice a week for 6 weeks) and their cardiac function was analyzed by echocardiography. Histological studies and Western blotting were performed to assess the severity and the mechanism of the underlying cardiac abnormality. Fisetin effectively suppressed calcineurin-NFATC3 involved cellular hypertrophic effects as observed in H9c2 cells and in SHR hearts. Fisetin also enhanced cardiac function and restored cardiac morphology and therefore, fisetin is a potential agent against hypertension associated hypertrophy and cardiac deterioration.
KW - 3,3′,4′,7-Tetrahydroxyflavone
KW - Cardiac remodeling
KW - Fisetin
KW - Flavonoid
KW - Hypertension
UR - http://www.scopus.com/inward/record.url?scp=85056638114&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85056638114&partnerID=8YFLogxK
U2 - 10.1016/j.jff.2018.10.038
DO - 10.1016/j.jff.2018.10.038
M3 - Article
AN - SCOPUS:85056638114
VL - 52
SP - 212
EP - 218
JO - Journal of Functional Foods
JF - Journal of Functional Foods
SN - 1756-4646
ER -