摘要

We present a bio-electrospinning technique for preparing cell-containing, oriented tubular scaffold intended for use as a nerve guide conduit (NGC). With a coaxial spinneret, solutions of PC-12 cells and poly(l-lactic acid) (PLLA) were delivered to the core and shell parts of the spinneret, respectively. When electrostatic field was applied, core/shell fiber was electrospun and collected. The electrospun fibers' morphology was characterized via scanning electron microscopy (SEM) and optical microscopy. Two structural characteristics were found: (1) the larger, hollow fibers had diameters in tens of micrometers and wall thicknesses around few micrometers, and (2) improved orientation in a preferred direction with the aid of a rotating collection device. Finally, fluorescent PC12 cells were found not only to exist and survive inside these hollow fibers, but also, upon addition of nerve growth factor, to undergo differentiation with neurite extended along the hollow fibers in the desired direction. All of these results demonstrate its potential application for NGC.
原文英語
頁(從 - 到)602-612
頁數11
期刊Textile Research Journal
82
發行號6
DOIs
出版狀態已發佈 - 四月 2012

指紋

Fibrous membranes
Electrospinning
Lactic acid
Lactic Acid
Fibers
Nerve Growth Factor
Scaffolds
Optical microscopy
Electric fields
Scanning electron microscopy

ASJC Scopus subject areas

  • Polymers and Plastics
  • Chemical Engineering (miscellaneous)

引用此文

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title = "Bio-electrospinning of poly(l-lactic acid) hollow fibrous membrane",
abstract = "We present a bio-electrospinning technique for preparing cell-containing, oriented tubular scaffold intended for use as a nerve guide conduit (NGC). With a coaxial spinneret, solutions of PC-12 cells and poly(l-lactic acid) (PLLA) were delivered to the core and shell parts of the spinneret, respectively. When electrostatic field was applied, core/shell fiber was electrospun and collected. The electrospun fibers' morphology was characterized via scanning electron microscopy (SEM) and optical microscopy. Two structural characteristics were found: (1) the larger, hollow fibers had diameters in tens of micrometers and wall thicknesses around few micrometers, and (2) improved orientation in a preferred direction with the aid of a rotating collection device. Finally, fluorescent PC12 cells were found not only to exist and survive inside these hollow fibers, but also, upon addition of nerve growth factor, to undergo differentiation with neurite extended along the hollow fibers in the desired direction. All of these results demonstrate its potential application for NGC.",
keywords = "bio-electrospinning, hollow fiber, nerve guide conduit, neurite, PC12, scaffold",
author = "Shih, {Yung Hsun} and Yang, {Jen Chang} and Li, {Su Han} and Yang, {Wei Chung Vivian} and Chen, {Chien Chung}",
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