Binding and Partial Denaturing of G-quartet DNA by Cdc13p of Saccharomyces cerevisiae

Yi Chien Lin, Jing Wen Shih, Chia Ling Hsu, Jing Jer Lin

研究成果: 雜誌貢獻文章同行評審

29 引文 斯高帕斯(Scopus)

摘要

The protein Cdc13p binds telomeres in vivo and is essential for the maintenance of the telomeres of Saccharomyces cerevisiae. In addition, Cdc13p is known to bind single-stranded TG1-3 DNA in vitro. Here we have shown that Cdc13p also binds DNA quadruplex, G-quartet, formed by TG 1-3 DNA. Moreover, the binding of Cde13p causes a partial denaturing of the G-quartet DNA. Formation of DNA quadruplexes may involve the intermolecular association of TG1-3 DNA and inhibit the extension of telomeres by telomerase. Thus, our finding suggests that Cde13p may disrupt telomere association and facilitate telomere replication.
原文英語
頁(從 - 到)47671-47674
頁數4
期刊Journal of Biological Chemistry
276
發行號50
DOIs
出版狀態已發佈 - 十二月 14 2001
對外發佈Yes

ASJC Scopus subject areas

  • Biochemistry

指紋 深入研究「Binding and Partial Denaturing of G-quartet DNA by Cdc13p of Saccharomyces cerevisiae」主題。共同形成了獨特的指紋。

引用此