Beta-adrenoceptor pathway enhances mitochondrial function in human neural stem cells via rotary cell culture system

Ming Chang Chiang, Heng Lin, Yi Chuan Cheng, Chia Hui Yen, Rong Nan Huang, Kuan Hung Lin

研究成果: 雜誌貢獻文章

22 引文 (Scopus)

摘要

The structure and function of the human nervous system are altered in space when compared with their state on earth. To investigate directly the influence of simulated microgravity conditions which may be beneficial for cultivation and proliferation of human neural stem cells (hNSCs), the rotary cell culture system (RCCS) developed at the National Aeronautics and Space Administration (NASA) was used. RCCS allows the creation of a unique microgravity environment of low shear force, high-mass transfer and enables three-dimensional (3D) cell culture of dissimilar cell types. The results show that simulated microgravity using an RCCS would induce β-adrenoceptor, upregulate cAMP formation and activate both PKA and CREB (cAMP response element binding protein) pathways. The expression of intracellular mitochondrial genes, including PGC1α (PPAR coactivator 1α), nuclear respiratory factors 1 and 2 (NRF1 and NRF2) and mitochondrial transcription factor A (Tfam), regulated by CREB, were all significantly increased at 72. h after the onset of microgravity. Accordingly and importantly, the ATP level and amount of mitochondrial mass were also increased. These results suggest that exposure to simulated microgravity using an RCCS would induce cellular proliferation in hNSCs via an increased mitochondrial function. In addition, the RCCS bioreactor would support hNSCs growth, which may have the potential for cell replacement therapy in neurological disorders.

原文英語
頁(從 - 到)130-136
頁數7
期刊Journal of Neuroscience Methods
207
發行號2
DOIs
出版狀態已發佈 - 六月 15 2012

指紋

Neural Stem Cells
Weightlessness
Adrenergic Receptors
Cell Culture Techniques
Cyclic AMP Response Element-Binding Protein
GA-Binding Protein Transcription Factor
Nuclear Respiratory Factor 1
United States National Aeronautics and Space Administration
Peroxisome Proliferator-Activated Receptors
Mitochondrial Genes
Bioreactors
Cell- and Tissue-Based Therapy
Nervous System Diseases
Nervous System
Up-Regulation
Adenosine Triphosphate
Cell Proliferation
Growth

ASJC Scopus subject areas

  • Neuroscience(all)

引用此文

Beta-adrenoceptor pathway enhances mitochondrial function in human neural stem cells via rotary cell culture system. / Chiang, Ming Chang; Lin, Heng; Cheng, Yi Chuan; Yen, Chia Hui; Huang, Rong Nan; Lin, Kuan Hung.

於: Journal of Neuroscience Methods, 卷 207, 編號 2, 15.06.2012, p. 130-136.

研究成果: 雜誌貢獻文章

Chiang, Ming Chang ; Lin, Heng ; Cheng, Yi Chuan ; Yen, Chia Hui ; Huang, Rong Nan ; Lin, Kuan Hung. / Beta-adrenoceptor pathway enhances mitochondrial function in human neural stem cells via rotary cell culture system. 於: Journal of Neuroscience Methods. 2012 ; 卷 207, 編號 2. 頁 130-136.
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