Bee venom induces apoptosis through intracellular Ca 2+-modulated intrinsic death pathway in human bladder cancer cells

Siu Wan Ip, Yung Lin Chu, Chun Shu Yu, Po Yuan Chen, Heng Chien Ho, Jai Sing Yang, Hui Ying Huang, Fu Shin Chueh, Tung-Iuan Lai, Jing Gung Chung

研究成果: 雜誌貢獻文章同行評審

45 引文 斯高帕斯(Scopus)

摘要

Objectives: To focus on bee venom-induced apoptosis in human bladder cancer TSGH-8301 cells and to investigate its signaling pathway to ascertain whether intracellular calcium iron (Ca 2+) is involved in this effect. Methods: Bee venom-induced cytotoxic effects, productions of reactive oxygen species and Ca 2+ and the level of mitochondrial membrane potential (ΔΨm) were analyzed by flow cytometry. Apoptosis-associated proteins were examined by Western blot analysis and confocal laser microscopy. Results: Bee venom-induced cell morphological changes and decreased cell viability through the induction of apoptosis in TSGH-8301 cell were found. Bee venom promoted the protein levels of Bax, caspase-9, caspase-3 and endonuclease G. The enhancements of endoplasmic reticulum stress-related protein levels were shown in bee venom-provoked apoptosis of TSGH-8301 cells. Bee venom promoted the activities of caspase-3, caspase-8, and caspase-9, increased Ca 2+ release and decreased the level of ΔΨm. Co-localization of immunofluorescence analysis showed the releases of endonuclease G and apoptosis-inducing factor trafficking to nuclei for bee venom-mediated apoptosis. The images revealed evidence of nuclear condensation and formation of apoptotic bodies by 4',6-diamidino-2-phenylindole staining and DNA gel electrophoresis showed the DNA fragmentation in TSGH-8301 cells. Conclusions: Bee venom treatment induces both caspase-dependent and caspase-independent apoptotic death through intracellular Ca 2+-modulated intrinsic death pathway in TSGH-8301 cells.
原文英語
頁(從 - 到)61-70
頁數10
期刊International Journal of Urology
19
發行號1
DOIs
出版狀態已發佈 - 一月 2012

ASJC Scopus subject areas

  • 泌尿科學

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