Baicalein Preventive Treatment Confers Optimal Cardioprotection by PTEN/Akt/NO Activation

Jing Li, Wei Tien Chang, Chang Qing Li, Chunpei Lee, Hsien Hao Huang, Chin Wan Hsu, Wen Jone Chen, Xiangdong Zhu, Chong Zhi Wang, Terry L. Vanden Hoek, Zuo Hui Shao

研究成果: 雜誌貢獻文章同行評審

13 引文 斯高帕斯(Scopus)

摘要

Baicalein is a flavonoid with excellent oxidant scavenging capability. It has been reported to protect against a variety of oxidative injuries including ischemia/reperfusion (I/R). However, the optimal treatment strategy for I/R injury and the protective mechanisms are not fully understood. In this study we employed an established chick cardiomyocyte model of I/R and investigated the effects of three baicalein treatment strategies on reactive oxygen species (ROS) scavenging, nitric oxide (NO) production and cell viability. The molecular signaling pathways were also explored. Compared to the I/R control (cell death 52.2±2.0%), baicalein preventive treatment (25μM, pretreated for 72h and continued through I/R) conferred the best protection (19.5±3.9%, p<0.001), followed by I/R treatment (treated during I/R) and reperfusion treatment (treated at reperfusion only). Preventive and I/R treatments almost completely abolished ROS generation during both ischemic and reperfusion phases, and increased NO production and Akt phosphorylation. Reperfusion treatment reduced the ROS burst in the early reperfusion phase only, and had no effect on NO production and Akt activation. Further, the phosphorylation of phosphatase and tensin homolog (PTEN), a phosphatase negatively regulating Akt activation, was significantly increased by baicalein preventive treatment and slightly by the I/R treatment. PTEN protein expression was reduced in the same trend accordingly. Baicalein reperfusion treatment had no effects on PTEN phosphorylation and expression. Our results indicate that baicalein preventive treatment confers optimal cardioprotection against I/R injury, and this protection involves effective oxidant scavenging and the activation of PTEN/Akt/NO pathway.
原文英語
頁(從 - 到)987-1001
頁數15
期刊American Journal of Chinese Medicine
45
發行號5
DOIs
出版狀態已發佈 - 1月 1 2017

ASJC Scopus subject areas

  • 補充和替代醫學

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