Autophagy promotes resistance to photodynamic therapy-induced apoptosis selectively in colorectal cancer stem-like cells

Ming Feng Wei, Min Wei Chen, Ke Cheng Chen, Pei Jen Lou, Susan Yun Fan Lin, Shih Chieh Hung, Michael Hsiao, Cheng Jung Yao, Ming Jium Shieh

研究成果: 雜誌貢獻文章同行評審

134 引文 斯高帕斯(Scopus)

摘要

Recent studies have indicated that cancer stem-like cells (CSCs) exhibit a high resistance to current therapeutic strategies, including photodynamic therapy (PDT), leading to the recurrence and progression of colorectal cancer (CRC). In cancer, autophagy acts as both a tumor suppressor and a tumor promoter. However, the role of autophagy in the resistance of CSCs to PDT has not been reported. In this study, CSCs were isolated from colorectal cancer cells using PROM1/CD133 (prominin 1) expression, which is a surface marker commonly found on stem cells of various tissues. We demonstrated that PpIX-mediated PDT induced the formation of autophagosomes in PROM1/CD133 + cells, accompanied by the upregulation of autophagy-related proteins ATG3, ATG5, ATG7, and ATG12. The inhibition of PDT-induced autophagy by pharmacological inhibitors and silencing of the ATG5 gene substantially triggered apoptosis of PROM1/CD133+ cells and decreased the ability of colonosphere formation in vitro and tumorigenicity in vivo. In conclusion, our results revealed a protective role played by autophagy against PDT in CSCs and indicated that targeting autophagy could be used to elevate the PDT sensitivity of CSCs. These findings would aid in the development of novel therapeutic approaches for CSC treatment.

原文英語
頁(從 - 到)1179-1192
頁數14
期刊Autophagy
10
發行號7
DOIs
出版狀態已發佈 - 7月 2014

ASJC Scopus subject areas

  • 分子生物學
  • 細胞生物學

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