Autophagy facilitates cytokine-induced ICAM-1 expression

Chi-Yun Wang, Tzu-Hui Chiang, Chia-Ling Chen, Po-Chun Tseng, Shun-Yi Chien, Yi-Jui Chuang, Tsan-Tzu Yang, Chia-Yuan Hsieh, Pui-Ching Choi, Chiou Feng Lin

研究成果: 雜誌貢獻文章

12 引文 (Scopus)

摘要

ICAM-1 can be induced by inflammatory cytokines such as IFN-γ and TNF-α. This study investigated whether autophagy regulates ICAM-1 given that autophagy facilitates signaling of these two cytokines. Exogenous IFN-γ induced ICAM-1 in human lung epithelial A549 cells carrying wild type p53, a transcription factor reported for ICAM-1, but not in PC14PE6/AS2 (AS2) cells carrying mutated p53. However, IFN-γ also induced ICAM-1 in A549 cells with short hairpin RNA-silenced p53. No changes in IFN-γ receptor expression were observed in AS2 cells, but IFN-γ-activated Jak2/STAT1/IFN regulatory factor 1 was markedly decreased. In AS2 cells, increased levels of reactive oxygen species induced the activation of Src homology domain-containing phosphatase 2 (SHP2), while SHP2 was essential for IFN-γ resistance. AS2 cells showed autophagy resistance, and the manipulation of the autophagy pathway altered IFN-γ resistance. Aberrant Bcl-2 expression and mammalian target of rapamycin activation contributed to both autophagy resistance and IFN-γ resistance. Autophagy, but not p53, also modulated TNF-α-induced NF-κB activation and ICAM-1 expression. Inhibiting autophagy decreased the adhesion of human monocytic U937 cells to IFN-γ-treated A549 cells. These results demonstrated that IFN-γ and TNF-α induced ICAM-1 expression through a common pathway that was regulated by autophagy, but not p53.
原文英語
頁(從 - 到)200-213
頁數14
期刊Innate Immunity
20
發行號2
DOIs
出版狀態已發佈 - 二月 2014
對外發佈Yes

指紋

Autophagy
Intercellular Adhesion Molecule-1
Cytokines
SH2 Domain-Containing Protein Tyrosine Phosphatases
U937 Cells
src Homology Domains
Sirolimus
Phosphoric Monoester Hydrolases
Small Interfering RNA
Reactive Oxygen Species
Transcription Factors
Epithelial Cells
Lung

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Immunology
  • Microbiology
  • Infectious Diseases

引用此文

Autophagy facilitates cytokine-induced ICAM-1 expression. / Wang, Chi-Yun; Chiang, Tzu-Hui; Chen, Chia-Ling; Tseng, Po-Chun; Chien, Shun-Yi; Chuang, Yi-Jui; Yang, Tsan-Tzu; Hsieh, Chia-Yuan; Choi, Pui-Ching; Lin, Chiou Feng.

於: Innate Immunity, 卷 20, 編號 2, 02.2014, p. 200-213.

研究成果: 雜誌貢獻文章

Wang, C-Y, Chiang, T-H, Chen, C-L, Tseng, P-C, Chien, S-Y, Chuang, Y-J, Yang, T-T, Hsieh, C-Y, Choi, P-C & Lin, CF 2014, 'Autophagy facilitates cytokine-induced ICAM-1 expression', Innate Immunity, 卷 20, 編號 2, 頁 200-213. https://doi.org/10.1177/1753425913488227
Wang, Chi-Yun ; Chiang, Tzu-Hui ; Chen, Chia-Ling ; Tseng, Po-Chun ; Chien, Shun-Yi ; Chuang, Yi-Jui ; Yang, Tsan-Tzu ; Hsieh, Chia-Yuan ; Choi, Pui-Ching ; Lin, Chiou Feng. / Autophagy facilitates cytokine-induced ICAM-1 expression. 於: Innate Immunity. 2014 ; 卷 20, 編號 2. 頁 200-213.
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AB - ICAM-1 can be induced by inflammatory cytokines such as IFN-γ and TNF-α. This study investigated whether autophagy regulates ICAM-1 given that autophagy facilitates signaling of these two cytokines. Exogenous IFN-γ induced ICAM-1 in human lung epithelial A549 cells carrying wild type p53, a transcription factor reported for ICAM-1, but not in PC14PE6/AS2 (AS2) cells carrying mutated p53. However, IFN-γ also induced ICAM-1 in A549 cells with short hairpin RNA-silenced p53. No changes in IFN-γ receptor expression were observed in AS2 cells, but IFN-γ-activated Jak2/STAT1/IFN regulatory factor 1 was markedly decreased. In AS2 cells, increased levels of reactive oxygen species induced the activation of Src homology domain-containing phosphatase 2 (SHP2), while SHP2 was essential for IFN-γ resistance. AS2 cells showed autophagy resistance, and the manipulation of the autophagy pathway altered IFN-γ resistance. Aberrant Bcl-2 expression and mammalian target of rapamycin activation contributed to both autophagy resistance and IFN-γ resistance. Autophagy, but not p53, also modulated TNF-α-induced NF-κB activation and ICAM-1 expression. Inhibiting autophagy decreased the adhesion of human monocytic U937 cells to IFN-γ-treated A549 cells. These results demonstrated that IFN-γ and TNF-α induced ICAM-1 expression through a common pathway that was regulated by autophagy, but not p53.

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