Autophagic reliance promotes metabolic reprogramming in oncogenic KRAS-driven tumorigenesis

H. Helen Lin, Yiyin Chung, Chun Ting Cheng, Ching Ouyang, Yong Fu, Ching Ying Kuo, Kevin K. Chi, Maryam Sadeghi, Peiguo Chu, Hsing Jien Kung, Chien Feng Li, Kirsten H. Limesand, David K. Ann

研究成果: 雜誌貢獻文章

6 引文 (Scopus)

摘要

Defects in basal autophagy limit the nutrient supply from recycling of intracellular constituents. Despite our understanding of the prosurvival role of macroautophagy/autophagy, how nutrient deprivation, caused by compromised autophagy, affects oncogenic KRAS-driven tumor progression is poorly understood. Here, we demonstrate that conditional impairment of the autophagy gene Atg5 (atg5-KO) extends the survival of KRASG12V-driven tumor-bearing mice by 38%. atg5-KO tumors spread more slowly during late tumorigenesis, despite a faster onset. atg5-KO tumor cells displayed reduced mitochondrial function and increased mitochondrial fragmentation. Metabolite profiles indicated a deficiency in the nonessential amino acid asparagine despite a compensatory overexpression of ASNS (asparagine synthetase), key enzyme for de novo asparagine synthesis. Inhibition of either autophagy or ASNS reduced KRASG12V-driven tumor cell proliferation, migration, and invasion, which was rescued by asparagine supplementation or knockdown of MFF (mitochondrial fission factor). Finally, these observations were reflected in human cancer-derived data, linking ASNS overexpression with poor clinical outcome in multiple cancers. Together, our data document a widespread yet specific asparagine homeostasis control by autophagy and ASNS, highlighting the previously unrecognized role of autophagy in suppressing the metabolic barriers of low asparagine and excessive mitochondrial fragmentation to permit malignant KRAS-driven tumor progression.
原文英語
頁(從 - 到)1481-1498
頁數18
期刊Autophagy
14
發行號9
DOIs
出版狀態已發佈 - 九月 2 2018

指紋

Autophagy
Carcinogenesis
Aspartate-Ammonia Ligase
Asparagine
Neoplasms
Mitochondrial Dynamics
Food
Recycling
Cell Movement
Homeostasis
Cell Proliferation
Amino Acids
Survival
Enzymes
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

引用此文

Lin, H. H., Chung, Y., Cheng, C. T., Ouyang, C., Fu, Y., Kuo, C. Y., ... Ann, D. K. (2018). Autophagic reliance promotes metabolic reprogramming in oncogenic KRAS-driven tumorigenesis. Autophagy, 14(9), 1481-1498. https://doi.org/10.1080/15548627.2018.1450708

Autophagic reliance promotes metabolic reprogramming in oncogenic KRAS-driven tumorigenesis. / Lin, H. Helen; Chung, Yiyin; Cheng, Chun Ting; Ouyang, Ching; Fu, Yong; Kuo, Ching Ying; Chi, Kevin K.; Sadeghi, Maryam; Chu, Peiguo; Kung, Hsing Jien; Li, Chien Feng; Limesand, Kirsten H.; Ann, David K.

於: Autophagy, 卷 14, 編號 9, 02.09.2018, p. 1481-1498.

研究成果: 雜誌貢獻文章

Lin, HH, Chung, Y, Cheng, CT, Ouyang, C, Fu, Y, Kuo, CY, Chi, KK, Sadeghi, M, Chu, P, Kung, HJ, Li, CF, Limesand, KH & Ann, DK 2018, 'Autophagic reliance promotes metabolic reprogramming in oncogenic KRAS-driven tumorigenesis', Autophagy, 卷 14, 編號 9, 頁 1481-1498. https://doi.org/10.1080/15548627.2018.1450708
Lin, H. Helen ; Chung, Yiyin ; Cheng, Chun Ting ; Ouyang, Ching ; Fu, Yong ; Kuo, Ching Ying ; Chi, Kevin K. ; Sadeghi, Maryam ; Chu, Peiguo ; Kung, Hsing Jien ; Li, Chien Feng ; Limesand, Kirsten H. ; Ann, David K. / Autophagic reliance promotes metabolic reprogramming in oncogenic KRAS-driven tumorigenesis. 於: Autophagy. 2018 ; 卷 14, 編號 9. 頁 1481-1498.
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abstract = "Defects in basal autophagy limit the nutrient supply from recycling of intracellular constituents. Despite our understanding of the prosurvival role of macroautophagy/autophagy, how nutrient deprivation, caused by compromised autophagy, affects oncogenic KRAS-driven tumor progression is poorly understood. Here, we demonstrate that conditional impairment of the autophagy gene Atg5 (atg5-KO) extends the survival of KRASG12V-driven tumor-bearing mice by 38{\%}. atg5-KO tumors spread more slowly during late tumorigenesis, despite a faster onset. atg5-KO tumor cells displayed reduced mitochondrial function and increased mitochondrial fragmentation. Metabolite profiles indicated a deficiency in the nonessential amino acid asparagine despite a compensatory overexpression of ASNS (asparagine synthetase), key enzyme for de novo asparagine synthesis. Inhibition of either autophagy or ASNS reduced KRASG12V-driven tumor cell proliferation, migration, and invasion, which was rescued by asparagine supplementation or knockdown of MFF (mitochondrial fission factor). Finally, these observations were reflected in human cancer-derived data, linking ASNS overexpression with poor clinical outcome in multiple cancers. Together, our data document a widespread yet specific asparagine homeostasis control by autophagy and ASNS, highlighting the previously unrecognized role of autophagy in suppressing the metabolic barriers of low asparagine and excessive mitochondrial fragmentation to permit malignant KRAS-driven tumor progression.",
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AU - Kuo, Ching Ying

AU - Chi, Kevin K.

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