Attenuative effects of platelet-rich plasma on 30 kDa fibronectin fragment-induced MMP-13 expression associated with TLR2 signaling in osteoarthritic chondrocytes and synovial fibroblasts

Hsien Tsung Lu, Jeng Wei Lu, Chian Her Lee, Yi Jen Peng, Herng Sheng Lee, You Hsiang Chu, Yi Jung Ho, Feng Cheng Liu, Pei Hung Shen, Chih Chien Wang

研究成果: 雜誌貢獻文章同行評審

摘要

Proteolytic fragments of fibronectin can have catabolic effects on cartilage, menisci, and synovium. Previous studies have reported that Toll-like receptor (TLR) signaling pathways might be associated with joint inflammation and joint destruction. Platelet-rich plasma (PRP) is increas-ingly being used to treat a range of joint conditions; however, it has yet to be determined whether PRP influences fibronectin fragment (FN-f) procatabolic activity and TLRs. In this study, human primary culture cells were treated with 30 kDa FN-f with/without PRP co-incubation, and then an-alyzed using real-time PCR to determine gene expression levels in articular chondrocytes, meniscal fibrochondrocytes, and synovial fibroblasts. Protein levels were evaluated by Western immunob-lotting. This study observed an increase in the protein expression of matrix metalloproteinases (MMPs), Toll-like receptor 2 (TLR2), nitric oxide synthase 2 (NOS2), prostaglandin-endoperoxide synthase (PTGS2), and cyclooxygenase 2 (COX2) in articular chondrocytes, meniscal fibrochondro-cytes, and synovial fibroblasts following insult with 30 kDa FN-f. Upregulation of these genes was significantly attenuated by PRP treatment. TLR2 and matrix metalloproteinase 13 (MMP-13) were also significantly attenuated by cotreatment with 30 kDa FN-f + PRP + TLR2 inhibitor. PRP treatment was shown to attenuate the 30 kDa FN-f-induced MMP-13 expression associated with the decreased expression of TLR2 in osteoarthritic chondrocytes and synovial fibroblasts. PRP treatment was also shown to attenuate procatabolic activity associated with MMP-13 expression via the TLR2 signaling pathway.
原文英語
文章編號4496
期刊Journal of Clinical Medicine
10
發行號19
DOIs
出版狀態已發佈 - 10月 1 2021

ASJC Scopus subject areas

  • 醫藥 (全部)

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