AT base pairs are less stable than GC base pairs in Z-DNA: The crystal structure of d(m5CGTAm5CG)

Andrew H.J. Wang, Toshio Hakoshima, Gijs van der Marel, Jacques H. van Boom, Alexander Rich

研究成果: 雜誌貢獻文章同行評審

150 引文 斯高帕斯(Scopus)

摘要

Two hexanucleoside pentaphosphates, 5-methyl and 5-bromo cytosine derivatives of d(CpGpTp-ApCpG) have been synthesized, crystallized, and their three-dimensional structure solved. They both form left-handed Z-DNA and the methylated derivative has been refined to 1.2 Å resolution. These are the first crystal Z-DNA structures that contain AT base pairs. The overall form of the molecule is very similar to that of the unmethylated or the fully methylated (dC-dG)3 hexamer although there are slight changes in base stacking. However, significant differences are found in the hydration of the helical groove. When GC base pairs are present, the helical groove is systematically filled with two water molecules per base pair hydrogen bonded to the bases. Both of these water molecules are not seen in the electron density map in the segments of the helix containing AT base pairs, probably because of solvent disorder. This could be one of the features that makes AT base pairs form Z-DNA less readily than GC base pairs.

原文英語
頁(從 - 到)321-331
頁數11
期刊Cell
37
發行號1
DOIs
出版狀態已發佈 - 5月 1984
對外發佈

ASJC Scopus subject areas

  • 生物化學、遺傳與分子生物學 (全部)

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