Association of blood active matrix metalloproteinase-3 with carotid plaque score from a community population in Taiwan

Li Ming Lien, Yi Chen Hsieh, Chyi Huey Bai, Wei-Hung Chen, Hou Chang Chiu, Fang I. Hsieh, Kou-Gi Shyu, Hung Yi Chiou, Chung-Yi Hsu

研究成果: 雜誌貢獻文章

16 引文 (Scopus)

摘要

Objective: Matrix metalloproteinases (MMPs) are implicated in the pathogenesis of atherosclerosis. However, the relationship between blood levels of MMPs and the extent of carotid atherosclerosis remains uncertain. We assessed blood levels of active MMPs in relation to the extent of carotid plaque formation and intima-media thickness (IMT) in a community population in Taiwan. Methods: In 433 subjects from a community primary stroke prevention program, blood levels of active MMP-1, total and active MMP-3 and active MMP-9 were determined using enzyme-linked immunosorbent assays, carotid plaque score (PS) and IMT by high-resolution B-mode ultrasonography and the common MMP-1, MMP-3, and MMP-9 promoter low- and high-activity genotypes by polymerase chain reaction and restriction fragment length polymorphism. Results: Study subjects were separated into 3 groups based on PS: group 1 (PS. = 0), group 2 (PS. = 1-2) and group 3 (PS ≥ 3). Blood levels of active and total MMP-3 bear a highly significant relationship with PS (both p<0.0001). A multiple ordinal logistic regression analysis revealed that blood levels of active MMP-3 are correlated with PS (OR, 1.4; 95% CI, 1.1-1.8; p= 0.0038) but not IMT. MMP-3-1612 6A6A is a dominant genotype in this population, which is associated with higher levels of blood active MMP-3. Conclusion: Blood levels of active MMP-3 are associated with the extent of carotid atherosclerosis based on PS but not IMT in this community population in Taiwan and the MMP-3-1612 6A6A genotype is associated with higher levels of blood active MMP-3.
原文英語
頁(從 - 到)595-600
頁數6
期刊Atherosclerosis
212
發行號2
DOIs
出版狀態已發佈 - 十月 2010

指紋

Matrix Metalloproteinase 3
Taiwan
Population
Matrix Metalloproteinases
Matrix Metalloproteinase 1
Carotid Artery Diseases
Genotype
Matrix Metalloproteinase 9
Primary Prevention
Restriction Fragment Length Polymorphisms
Ultrasonography
Atherosclerosis
Logistic Models
Enzyme-Linked Immunosorbent Assay
Stroke
Regression Analysis
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

引用此文

Association of blood active matrix metalloproteinase-3 with carotid plaque score from a community population in Taiwan. / Lien, Li Ming; Hsieh, Yi Chen; Bai, Chyi Huey; Chen, Wei-Hung; Chiu, Hou Chang; Hsieh, Fang I.; Shyu, Kou-Gi; Chiou, Hung Yi; Hsu, Chung-Yi.

於: Atherosclerosis, 卷 212, 編號 2, 10.2010, p. 595-600.

研究成果: 雜誌貢獻文章

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title = "Association of blood active matrix metalloproteinase-3 with carotid plaque score from a community population in Taiwan",
abstract = "Objective: Matrix metalloproteinases (MMPs) are implicated in the pathogenesis of atherosclerosis. However, the relationship between blood levels of MMPs and the extent of carotid atherosclerosis remains uncertain. We assessed blood levels of active MMPs in relation to the extent of carotid plaque formation and intima-media thickness (IMT) in a community population in Taiwan. Methods: In 433 subjects from a community primary stroke prevention program, blood levels of active MMP-1, total and active MMP-3 and active MMP-9 were determined using enzyme-linked immunosorbent assays, carotid plaque score (PS) and IMT by high-resolution B-mode ultrasonography and the common MMP-1, MMP-3, and MMP-9 promoter low- and high-activity genotypes by polymerase chain reaction and restriction fragment length polymorphism. Results: Study subjects were separated into 3 groups based on PS: group 1 (PS. = 0), group 2 (PS. = 1-2) and group 3 (PS ≥ 3). Blood levels of active and total MMP-3 bear a highly significant relationship with PS (both p<0.0001). A multiple ordinal logistic regression analysis revealed that blood levels of active MMP-3 are correlated with PS (OR, 1.4; 95{\%} CI, 1.1-1.8; p= 0.0038) but not IMT. MMP-3-1612 6A6A is a dominant genotype in this population, which is associated with higher levels of blood active MMP-3. Conclusion: Blood levels of active MMP-3 are associated with the extent of carotid atherosclerosis based on PS but not IMT in this community population in Taiwan and the MMP-3-1612 6A6A genotype is associated with higher levels of blood active MMP-3.",
keywords = "Blood active matrix metalloproteinase-3, Carotid plaque, MMP-3 genotype",
author = "Lien, {Li Ming} and Hsieh, {Yi Chen} and Bai, {Chyi Huey} and Wei-Hung Chen and Chiu, {Hou Chang} and Hsieh, {Fang I.} and Kou-Gi Shyu and Chiou, {Hung Yi} and Chung-Yi Hsu",
year = "2010",
month = "10",
doi = "10.1016/j.atherosclerosis.2010.05.040",
language = "English",
volume = "212",
pages = "595--600",
journal = "Atherosclerosis",
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TY - JOUR

T1 - Association of blood active matrix metalloproteinase-3 with carotid plaque score from a community population in Taiwan

AU - Lien, Li Ming

AU - Hsieh, Yi Chen

AU - Bai, Chyi Huey

AU - Chen, Wei-Hung

AU - Chiu, Hou Chang

AU - Hsieh, Fang I.

AU - Shyu, Kou-Gi

AU - Chiou, Hung Yi

AU - Hsu, Chung-Yi

PY - 2010/10

Y1 - 2010/10

N2 - Objective: Matrix metalloproteinases (MMPs) are implicated in the pathogenesis of atherosclerosis. However, the relationship between blood levels of MMPs and the extent of carotid atherosclerosis remains uncertain. We assessed blood levels of active MMPs in relation to the extent of carotid plaque formation and intima-media thickness (IMT) in a community population in Taiwan. Methods: In 433 subjects from a community primary stroke prevention program, blood levels of active MMP-1, total and active MMP-3 and active MMP-9 were determined using enzyme-linked immunosorbent assays, carotid plaque score (PS) and IMT by high-resolution B-mode ultrasonography and the common MMP-1, MMP-3, and MMP-9 promoter low- and high-activity genotypes by polymerase chain reaction and restriction fragment length polymorphism. Results: Study subjects were separated into 3 groups based on PS: group 1 (PS. = 0), group 2 (PS. = 1-2) and group 3 (PS ≥ 3). Blood levels of active and total MMP-3 bear a highly significant relationship with PS (both p<0.0001). A multiple ordinal logistic regression analysis revealed that blood levels of active MMP-3 are correlated with PS (OR, 1.4; 95% CI, 1.1-1.8; p= 0.0038) but not IMT. MMP-3-1612 6A6A is a dominant genotype in this population, which is associated with higher levels of blood active MMP-3. Conclusion: Blood levels of active MMP-3 are associated with the extent of carotid atherosclerosis based on PS but not IMT in this community population in Taiwan and the MMP-3-1612 6A6A genotype is associated with higher levels of blood active MMP-3.

AB - Objective: Matrix metalloproteinases (MMPs) are implicated in the pathogenesis of atherosclerosis. However, the relationship between blood levels of MMPs and the extent of carotid atherosclerosis remains uncertain. We assessed blood levels of active MMPs in relation to the extent of carotid plaque formation and intima-media thickness (IMT) in a community population in Taiwan. Methods: In 433 subjects from a community primary stroke prevention program, blood levels of active MMP-1, total and active MMP-3 and active MMP-9 were determined using enzyme-linked immunosorbent assays, carotid plaque score (PS) and IMT by high-resolution B-mode ultrasonography and the common MMP-1, MMP-3, and MMP-9 promoter low- and high-activity genotypes by polymerase chain reaction and restriction fragment length polymorphism. Results: Study subjects were separated into 3 groups based on PS: group 1 (PS. = 0), group 2 (PS. = 1-2) and group 3 (PS ≥ 3). Blood levels of active and total MMP-3 bear a highly significant relationship with PS (both p<0.0001). A multiple ordinal logistic regression analysis revealed that blood levels of active MMP-3 are correlated with PS (OR, 1.4; 95% CI, 1.1-1.8; p= 0.0038) but not IMT. MMP-3-1612 6A6A is a dominant genotype in this population, which is associated with higher levels of blood active MMP-3. Conclusion: Blood levels of active MMP-3 are associated with the extent of carotid atherosclerosis based on PS but not IMT in this community population in Taiwan and the MMP-3-1612 6A6A genotype is associated with higher levels of blood active MMP-3.

KW - Blood active matrix metalloproteinase-3

KW - Carotid plaque

KW - MMP-3 genotype

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U2 - 10.1016/j.atherosclerosis.2010.05.040

DO - 10.1016/j.atherosclerosis.2010.05.040

M3 - Article

C2 - 20609440

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VL - 212

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EP - 600

JO - Atherosclerosis

JF - Atherosclerosis

SN - 0021-9150

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