Association of a functional polymorphism in the promoter region of TLR-3 with osteoarthritis: A two-stage case-control study

Hsin Yi Yang, Herng Sheng Lee, Chian Her Lee, Wen Hui Fang, Hsiang Cheng Chen, Donald M. Salter, Sui Lung Su

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30 引文 斯高帕斯(Scopus)

摘要

Recent studies have suggested that polymorphisms in toll-like receptor 9 (TLR-9), an endosomal TLR, are associated with knee osteoarthritis (OA). TLR-3, -7, and -8 are also found on the surface of endosomes and to investigate whether similar associations exist with polymorphisms in these TLR genes we performed a two-stage case-control study and genotyped 11 TLR single nucleotide polymorphisms (SNPs) in 823 OA cases and 594 healthy controls by polymerase chain reaction restriction fragment length polymorphism assays. Real-time PCR was performed to assess the functional expression of an identified promoter polymorphism in TLR-3 following dexamethasone stimulation of articular chondrocytes. An association between TLR-3 SNPs at rs3775296 and rs3775290 and OA was identified in both populations. In males the allelic frequencies of TLR-7 rs179010 and TLR-8 rs5744080 were significantly different between OA cases and healthy controls. The ATCA, CTCA, and CCTA haplotypes of TLR-3 were associated with OA susceptibility. A significant difference in TLR-3 gene expression following dexamethasone treatment was seen among the various genotypes of rs3775296 (p = 0.004). Our findings indicate that a SNP in the promoter region of TLR-3 is associated with elevated TLR-3 gene expression and susceptibility to knee OA in a Chinese Han population.

原文英語
頁(從 - 到)680-685
頁數6
期刊Journal of Orthopaedic Research
31
發行號5
DOIs
出版狀態已發佈 - 五月 2013

ASJC Scopus subject areas

  • 骨科和運動醫學

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