Assaying for structural variation in the parvovirus capsid and its role in infection

Wendy S. Weichert, John S L Parker, A. T M Wahid, Shwu Fen Chang, Ellen Meier, Colin R. Parrish

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73 引文 斯高帕斯(Scopus)

摘要

The capsid of canine parvovirus (CPV) was assayed for susceptibility to proteases and for structural variation. The natural cleavage of VP2 to VP3 in CPV full (DNA containing) particles recovered from tissue culture occurred within the sequence Arg-Asn-Glu-Arg ↓ Ala-Thr. Trypsin, chymotrypsin, bromelain, and cathepsin B all cleaved >90% of the VP2 to VP3 in full but not in empty capsids and did not digest the capsid further. Digestion with proteinase K, Pronase, papain, or subtilisin cleaved the VP2 to VP3 and also cleaved at additional internal sites, causing particle disintegration and protein degradation. Several partial digestion products produced by proteinase K or subtilisin were ~31-32.5 kDa, indicating cleavage within loop 3 of the capsid protein as well as other sites. Protease treatment of capsids at pH 5.5 or 7.5 did not significantly alter their susceptibility to digestion. The isoelectric point of CPV empty capsids was pH 5.3, and full capsids were 0.3 pH more acidic, but after proteolysis of VP2 to VP3, the pl of the full capsids became the same as that of the empty capsids. Antibodies against various capsid protein sequences showed the amino termini of most VP2 molecules were on the outside of full but not empty particles, that the VP1- unique sequence was internal, and that the capsid could be disintegrated by heat or urea treatment to expose the internal sequences. Capsids added to cells were localized within the cell cytoplasm in vesicles that appeared to be lysosomes. Microinjected capsids remained primarily in the cytoplasm, although a small proportion was observed to be in the nucleus after 2 h. After CPV capsids labeled with [35S]methionine were bound to cells at 0°C and the cells warmed, little cleavage of VP1 or VP2 was observed even after prolonged incubation. Inoculation of cells with virus in the presence of proteinase inhibitors did not significantly reduce the infection.

原文英語
頁(從 - 到)106-117
頁數12
期刊Virology
250
發行號1
DOIs
出版狀態已發佈 - 十月 10 1998
對外發佈Yes

    指紋

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

引用此

Weichert, W. S., Parker, J. S. L., Wahid, A. T. M., Chang, S. F., Meier, E., & Parrish, C. R. (1998). Assaying for structural variation in the parvovirus capsid and its role in infection. Virology, 250(1), 106-117. https://doi.org/10.1006/viro.1998.9352