Ascorbate supplement reduces oxidative stress in dyslipidemic patients undergoing apheresis

Chiang Ting Chien, Wei Tien Chang, Heui Wen Chen, Tzung Dau Wang, Shaw Yih Liou, Tzay Jinn Chen, Yen Lin Chang, Yuan Teh Lee, Su Ming Hsu

研究成果: 雜誌貢獻文章同行評審

33 引文 斯高帕斯(Scopus)

摘要

Objective-The effect of ascorbate treatment on apheresis-induced oxidative stress in uremic and dyslipidemic patients was evaluated. Methods and Results-We developed a chemiluminescence-emission spectrum and high-performance liquid chromatography analysis to assess the effect of ascorbate supplement on plasma reactive oxygen species (ROS) scavenging activity and oxidized lipid/protein production in hyperlipidemic and uremic patients undergoing apheresis. Apheresis was efficient in reduction of atherogenic lipoproteins, complement, fibrinogen, soluble intercellular adhesion molecule-1, and oxidative parameters including phosphatidylcholine hydroperoxide (PCOOH), malonaldehyde, methylguanidine, and diotyrosine. Apheresis itself, however, activated leukocytes to increase ROS activity and reduced the plasma ROS scavenging activity. Ascorbate administration selectively diminished apheresis-enhanced H2O2 and inflammatory mediators such as tumor necrosis factor alpha (TNF-α) and monocyte chemoattractant protein-1. Chronically dyslipidemic and uremic patients undergoing biweekly apheresis plus ascorbate treatment had lower levels of C-reactive protein and PCOOH than did those without ascorbate treatment during a 6-month follow-up study period. Conclusions-We demonstrate that apheresis with ascorbate treatment provides a therapeutic potential in reducing atherosclerotic risk via inhibition of H2O2-induced oxidative stress in patients with uremia or dyslipidemia.

原文英語
頁(從 - 到)1111-1117
頁數7
期刊Arteriosclerosis, Thrombosis, and Vascular Biology
24
發行號6
DOIs
出版狀態已發佈 - 六月 1 2004
對外發佈Yes

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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