Arsenic methylation capacity, body retention, and null genotypes of glutathione S-transferase M1 and T1 among current arsenic-exposed residents in Taiwan

Hung Yi Chiou, Yu Mei Hsueh, Ling Ling Hsieh, Ling I. Hsu, Hsu Yi-Hsiang, Fang I. Hsieh, Min Li Wei, Hsiang Chih Chen, Hui Ting Yang, Lan Chin Leu, Tzu Hsien Chu, Chuan Chen-Wu, Mo Hsiung Yang, Chien Jen Chen

研究成果: 雜誌貢獻文章

170 引文 斯高帕斯(Scopus)

摘要

In order to elucidate the relationships among arsenic methylation capacity, body retention, and genetic polymorphisms of glutathione S- transferase (GST) M1 and T1, a total of 115 study subjects were recruited from Lanyang Basin located on the northeast coast of Taiwan. Specimens of drinking water, blood, urine, hair and toenail were collected from each study subject. Urinary inorganic and methylated arsenic were speciated by high performance liquid chromatography combined with hydride generation atomic absorption spectrometry. Arsenic concentration in hair and toenail were quantitated by atomic absorption spectrophotometry. The polymerase chain reaction was used to determine genetic polymorphisms of GST M1 and T1. Arsenic concentrations in urine, hair, and toenail of study subjects were positively correlated with arsenic levels in their drinking water. Percentages of various arsenic species in urine (mean ± standard error (SE)) were 11.8 ± 1.0, 26.9 ± 1.2 and 61.3 ± 1.4, respectively, for inorganic arsenic, monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA). Men and women had similar arsenic methylation capability. No associations were observed between arsenic methylation capability and arsenic content in either drinking water or urine. Ratios of arsenic contents in hair and toenail to urinary arsenic contert (mean ± standard error) were 6.2 ± 0.7 and 16.5 ± 1.7, respectively. Genetic polymorphisms of GST MI and TI were significantly associated with arsenic methylation. Subjects having the null genotype of GST MI had an increased percentage of inorganic arsenic in urine, while those with null genotype of GST TI had an elevated percentage of DMA in urine. Arsenic contents in hair and toenail were significantly correlated with the increase in arsenic concentrations of drinking water and urine, while no significant associations were observed between arsenic contents in hair and toenail and polymorphisms of GST MI and TI. The relationship between arsenic methylation capability and body retention was modified by genetic polymorphisms of GST M1 and T1. Arsenic contents in hair and toenail were negatively associated with MMA percentage and positively associated with DMA percentage among subjects having null genotypes of GST VII and TI, but not among those with non null genotypes.
原文英語
頁(從 - 到)197-207
頁數11
期刊Mutation Research - Reviews in Mutation Research
386
發行號3
DOIs
出版狀態已發佈 - 六月 1997

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Keywords

  • Arsenic
  • Body retention
  • Glutathione S-transferase
  • Methylation

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis

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