Argon plasma surface modified porcine bone substitute improved osteoblast-like cell behavior

Cheuk Sing Choy, Eisner Salamanca, Pei Ying Lin, Haw Ming Huang, Nai Chia Teng, Yu Hwa Pan, Wei Jen Chang

研究成果: 雜誌貢獻文章

摘要

Low-temperature plasma-treated porcine grafts (PGPT) may be an effective means for treating demanding osseous defects and enhance our understanding of plasma-tissue engineering. We chemically characterized porcine grafts under low-temperature Argon plasma treatment (CAP) and evaluated their biocompatibility in-vitro. Our results showed that PGPT did not differ in roughness, dominant crystalline phases, absorption peaks corresponding to phosphate band peaks, or micro-meso pore size, compared to non-treated porcine grafts. The PGPT Ca/P ratio was 2.16; whereas the porcine control ratio was 2.04 (p < 0.05). PGPT's [C 1s], [P 2p] and [Ca 2p] values were 24.3%, 5.6% and 11.0%, respectively, indicating that PGPT was an apatite without another crystalline phase. Cell viability and alkaline phosphatase assays revealed enhanced proliferation and osteoblastic differentiation for the cells cultivated in the PGPT media after 5 days (p < 0.05). The cells cultured in PGPT medium had higher bone sialoprotein and osteocalcin relative mRNA expression compared to cells cultured in non-treated porcine grafts (p < 0.05). CAP treatment of porcine particles did not modify the biomaterial's surface and improved the proliferation and differentiation of osteoblast-like cells.
原文英語
頁(從 - 到)9-134
頁數126
期刊Coatings
9
發行號2
DOIs
出版狀態已發佈 - 一月 1 2019

指紋

Bone Substitutes
osteoblasts
Argon
Osteoblasts
argon plasma
cold plasmas
cultured cells
Grafts
bones
Bone
osteocalcin
substitutes
Plasmas
phosphatases
tissue engineering
biocompatibility
apatites
cells
viability
phosphates

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Surfaces and Interfaces

引用此文

Argon plasma surface modified porcine bone substitute improved osteoblast-like cell behavior. / Choy, Cheuk Sing; Salamanca, Eisner; Lin, Pei Ying; Huang, Haw Ming; Teng, Nai Chia; Pan, Yu Hwa; Chang, Wei Jen.

於: Coatings, 卷 9, 編號 2, 01.01.2019, p. 9-134.

研究成果: 雜誌貢獻文章

Choy, Cheuk Sing ; Salamanca, Eisner ; Lin, Pei Ying ; Huang, Haw Ming ; Teng, Nai Chia ; Pan, Yu Hwa ; Chang, Wei Jen. / Argon plasma surface modified porcine bone substitute improved osteoblast-like cell behavior. 於: Coatings. 2019 ; 卷 9, 編號 2. 頁 9-134.
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abstract = "Low-temperature plasma-treated porcine grafts (PGPT) may be an effective means for treating demanding osseous defects and enhance our understanding of plasma-tissue engineering. We chemically characterized porcine grafts under low-temperature Argon plasma treatment (CAP) and evaluated their biocompatibility in-vitro. Our results showed that PGPT did not differ in roughness, dominant crystalline phases, absorption peaks corresponding to phosphate band peaks, or micro-meso pore size, compared to non-treated porcine grafts. The PGPT Ca/P ratio was 2.16; whereas the porcine control ratio was 2.04 (p < 0.05). PGPT's [C 1s], [P 2p] and [Ca 2p] values were 24.3{\%}, 5.6{\%} and 11.0{\%}, respectively, indicating that PGPT was an apatite without another crystalline phase. Cell viability and alkaline phosphatase assays revealed enhanced proliferation and osteoblastic differentiation for the cells cultivated in the PGPT media after 5 days (p < 0.05). The cells cultured in PGPT medium had higher bone sialoprotein and osteocalcin relative mRNA expression compared to cells cultured in non-treated porcine grafts (p < 0.05). CAP treatment of porcine particles did not modify the biomaterial's surface and improved the proliferation and differentiation of osteoblast-like cells.",
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AU - Teng, Nai Chia

AU - Pan, Yu Hwa

AU - Chang, Wei Jen

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