APEG-1, a novel gene preferentially expressed in aortic smooth muscle cells, is down-regulated by vascular injury

Chung Ming Hsieh, Masao Yoshizumi, Wilson O. Endege, Choon Joo Kho, Mukesh K. Jain, Saori Kashiki, Rico De los Santos, Wen Sen Leet, Mark A. Perrella, Mu En Lee

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38 引文 斯高帕斯(Scopus)

摘要

Despite the importance of phenotypic alterations in arterial smooth muscle cells (ASMC) during the pathogenesis of arteriosclerosis, little is known about genes that define differentiated ASMC. Using differential mRNA display, we isolated a novel gene preferentially expressed in the rat aorta and termed this gene APEG-1. The cDNA of rat APEG-1 contained an open reading frame encoding 113 amino acids, which would predict a basic protein of 12.7 kDa. The amino acid sequence of rat APEG-1 was highly conserved among human and mouse homologues (97 and 98%, respectively). Using an APEG-1 fusion protein containing an N-terminal c-Myc tag, we identified APEG-1 as a nuclear protein. By in situ hybridization, APEG-1 mRNA was expressed in rat ASMC. Although APEG-1 was expressed highly in differentiated ASMC in vivo, its expression was quickly down-regulated and disappeared in dedifferentiated ASMC in culture. In vivo, APEG-1 mRNA levels decreased by more than 80% in response to vascular injury as ASMC changed from a quiescent to a proliferative phenotype. Taken together, these data indicate that APEG-1 is a novel marker for differentiated ASMC and may have a role in regulating growth and differentiation of this cell type.

原文英語
頁(從 - 到)17354-17359
頁數6
期刊Journal of Biological Chemistry
271
發行號29
DOIs
出版狀態已發佈 - 1996
對外發佈

ASJC Scopus subject areas

  • 生物化學
  • 分子生物學
  • 細胞生物學

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