Antrodia cinnamomea enhances chemo-sensitivity of 5-fu and suppresses colon tumorigenesis and cancer stemness via up-regulation of tumor suppressor miR-142-3p

Yan Jiun Huang, Vijesh Kumar Yadav, Prateeti Srivastava, Alexander T.H. Wu, Thanh Tuan Huynh, Po Li Wei, Chi Ying F. Huang, Tse Hung Huang

研究成果: 雜誌貢獻文章

1 引文 (Scopus)

摘要

5-Fluorouracil (5-FU) regimen remains the backbone of the first-line agent to treat colon cancer, but often these patients develop resistance. Cancer stem cells (CSC’s) are considered as one of the key contributors in the development of drug resistance and tumor recurrence. We aimed to provide preclinical evidence for Antrodia cinnamomea (AC), as a potential in suppressing colon cancer CSC’s to overcome 5-FU drug-resistant. In-vitro assays including cell viability, colony formation, AC + 5-FU drug combination index and tumor sphere generation were applied to determine the inhibitory effect of AC. Mouse xenograft models also incorporated to evaluate in vivo effect of AC. AC treatment significantly inhibited the proliferation, colony formation and tumor sphere generation. AC also inhibited the expression of oncogenic markers (NF-κB, and C-myc), EMT/metastasis markers (vimentin and MMP3) and stemness associated markers (β-catenin, SOX-2 and Nanog). Sequential treatment of AC and 5-FU synergized and reduces colon cancer viability both in vivo and in vitro. Mechanistically, AC mediated anti-tumor effect was associated with an increased level of tumor suppressor microRNAs especially, miR142-3p. AC can be a potent synergistic adjuvant, down-regulates cancer stemness genes and enhances the antitumor ability of 5-FU by stimulating apoptosis-associated genes, suppressing inflammation and metastasis genes through miR142-3p in colon cancer.

原文英語
文章編號306
期刊Biomolecules
9
發行號8
DOIs
出版狀態已發佈 - 八月 1 2019

指紋

Antrodia
Fluorouracil
Colonic Neoplasms
Tumors
Carcinogenesis
Up-Regulation
Genes
Neoplasms
Stem cells
Neoplastic Stem Cells
Catenins
Vimentin
Drug Combinations
MicroRNAs
Heterografts
Pharmaceutical Preparations
Assays
Neoplasm Metastasis
Cells
Apoptosis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

引用此文

Antrodia cinnamomea enhances chemo-sensitivity of 5-fu and suppresses colon tumorigenesis and cancer stemness via up-regulation of tumor suppressor miR-142-3p. / Huang, Yan Jiun; Yadav, Vijesh Kumar; Srivastava, Prateeti; Wu, Alexander T.H.; Huynh, Thanh Tuan; Wei, Po Li; Huang, Chi Ying F.; Huang, Tse Hung.

於: Biomolecules, 卷 9, 編號 8, 306, 01.08.2019.

研究成果: 雜誌貢獻文章

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title = "Antrodia cinnamomea enhances chemo-sensitivity of 5-fu and suppresses colon tumorigenesis and cancer stemness via up-regulation of tumor suppressor miR-142-3p",
abstract = "5-Fluorouracil (5-FU) regimen remains the backbone of the first-line agent to treat colon cancer, but often these patients develop resistance. Cancer stem cells (CSC’s) are considered as one of the key contributors in the development of drug resistance and tumor recurrence. We aimed to provide preclinical evidence for Antrodia cinnamomea (AC), as a potential in suppressing colon cancer CSC’s to overcome 5-FU drug-resistant. In-vitro assays including cell viability, colony formation, AC + 5-FU drug combination index and tumor sphere generation were applied to determine the inhibitory effect of AC. Mouse xenograft models also incorporated to evaluate in vivo effect of AC. AC treatment significantly inhibited the proliferation, colony formation and tumor sphere generation. AC also inhibited the expression of oncogenic markers (NF-κB, and C-myc), EMT/metastasis markers (vimentin and MMP3) and stemness associated markers (β-catenin, SOX-2 and Nanog). Sequential treatment of AC and 5-FU synergized and reduces colon cancer viability both in vivo and in vitro. Mechanistically, AC mediated anti-tumor effect was associated with an increased level of tumor suppressor microRNAs especially, miR142-3p. AC can be a potent synergistic adjuvant, down-regulates cancer stemness genes and enhances the antitumor ability of 5-FU by stimulating apoptosis-associated genes, suppressing inflammation and metastasis genes through miR142-3p in colon cancer.",
keywords = "5-FU, AC, Colon cancer, EMT, miR-142-3p, Stemness",
author = "Huang, {Yan Jiun} and Yadav, {Vijesh Kumar} and Prateeti Srivastava and Wu, {Alexander T.H.} and Huynh, {Thanh Tuan} and Wei, {Po Li} and Huang, {Chi Ying F.} and Huang, {Tse Hung}",
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AU - Huang, Yan Jiun

AU - Yadav, Vijesh Kumar

AU - Srivastava, Prateeti

AU - Wu, Alexander T.H.

AU - Huynh, Thanh Tuan

AU - Wei, Po Li

AU - Huang, Chi Ying F.

AU - Huang, Tse Hung

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AB - 5-Fluorouracil (5-FU) regimen remains the backbone of the first-line agent to treat colon cancer, but often these patients develop resistance. Cancer stem cells (CSC’s) are considered as one of the key contributors in the development of drug resistance and tumor recurrence. We aimed to provide preclinical evidence for Antrodia cinnamomea (AC), as a potential in suppressing colon cancer CSC’s to overcome 5-FU drug-resistant. In-vitro assays including cell viability, colony formation, AC + 5-FU drug combination index and tumor sphere generation were applied to determine the inhibitory effect of AC. Mouse xenograft models also incorporated to evaluate in vivo effect of AC. AC treatment significantly inhibited the proliferation, colony formation and tumor sphere generation. AC also inhibited the expression of oncogenic markers (NF-κB, and C-myc), EMT/metastasis markers (vimentin and MMP3) and stemness associated markers (β-catenin, SOX-2 and Nanog). Sequential treatment of AC and 5-FU synergized and reduces colon cancer viability both in vivo and in vitro. Mechanistically, AC mediated anti-tumor effect was associated with an increased level of tumor suppressor microRNAs especially, miR142-3p. AC can be a potent synergistic adjuvant, down-regulates cancer stemness genes and enhances the antitumor ability of 5-FU by stimulating apoptosis-associated genes, suppressing inflammation and metastasis genes through miR142-3p in colon cancer.

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