Antisense RNA to inducible nitric oxide synthase reduces cytokine-mediated brain endothelial cell death

Ding I. Yang, Shawei Chen, Uthayashanker R. Ezekiel, Jan Xu, Yingji Wu, Chung Y. Hsu

研究成果: 雜誌貢獻文章同行評審

5 引文 斯高帕斯(Scopus)

摘要

We test whether inhibition of inducible nitric oxide synthase (iNOS) can exert a cytoprotective effect on cerebral endothelial cells upon stimulation by pro-inflammatory cytokines. Mouse brain endothelial cells were stably transfected to express an antisense RNA against iNOS driven by an endothelium-specific von Willebrand factor (vWF) promoter. Upon stimulation with tumor necrosis factor-α (TNF-α) plus interferon-γ (IFN-γ), antisense transfectants showed less iNOS enzymatic activity with less nitric oxide (NO) when compared to the sense control cells. Correspondingly, the antisense cells showed a reduced LDH release and less cytosolic content of oligonucleosomes. These findings establish a cell-specific antisense strategy and confirm the cytotoxic role of iNOS expression in cultured cerebral endothelial cells.
原文英語
頁(從 - 到)439-447
頁數9
期刊Annals of the New York Academy of Sciences
1042
DOIs
出版狀態已發佈 - 2005

ASJC Scopus subject areas

  • 生物化學、遺傳與分子生物學 (全部)
  • 科學史與哲學

指紋

深入研究「Antisense RNA to inducible nitric oxide synthase reduces cytokine-mediated brain endothelial cell death」主題。共同形成了獨特的指紋。

引用此