Antiproliferative effect of isosteviol on angiotensin-II-treated rat aortic smooth muscle cells

Kar Lok Wong, Jia Wei Lin, Ju Chi Liu, Hung Yu Yang, Pei Feng Kao, Cheng Hsien Chen, Shih Hurng Loh, Wen Ta Chiu, Tzu-Hurng Cheng, Jaung Geng Lin, Hong Jye Hong

研究成果: 雜誌貢獻文章

31 引文 (Scopus)

摘要

Isosteviol is a derivative of stevioside, a constituent of Stevia rebaudiana, which is commonly used as a noncaloric sugar substitute in Japan and Brazil. The aims of this study were to examine whether isosteviol alters angiotensin-II-induced cell proliferation in rat aortic smooth muscle cells. Cultured rat aortic smooth muscle cells were preincubated with isosteviol, then stimulated with angiotensin II, after which [3H]thymidine incorporation and endothelin-1 secretion were examined. Isosteviol (1-100 μmol/l) inhibits angiotensin-II-induced DNA synthesis and endothelin-1 secretion. Measurements of 2′7′-dichlorofluorescin diacetate, a redox-sensitive fluorescent dye, showed an isosteviol-mediated inhibition of intracellular reactive oxygen species generated by the effects of angiotensin II. The inductive properties of angiotensin II on extracellular signal-regulated kinase (ERK) phosphorylation were found reversed with isosteviol and antioxidants such as N-acetylcysteine. In summary, we speculate that isosteviol inhibits angiotensin-II-induced cell proliferation and endothelin-1 secretion via attenuation of reactive oxygen species generation. Thus, this study provides important insights that may contribute to the effects of isosteviol on the cardiovascular system.
原文英語
頁(從 - 到)163-169
頁數7
期刊Pharmacology
76
發行號4
DOIs
出版狀態已發佈 - 四月 2006

指紋

Angiotensin II
Smooth Muscle Myocytes
Endothelin-1
Reactive Oxygen Species
Stevia
Cell Proliferation
Sweetening Agents
isosteviol
Extracellular Signal-Regulated MAP Kinases
Acetylcysteine
Cardiovascular System
Fluorescent Dyes
Thymidine
Oxidation-Reduction
Brazil
Japan
Antioxidants
Phosphorylation
DNA

ASJC Scopus subject areas

  • Pharmacology

引用此文

Antiproliferative effect of isosteviol on angiotensin-II-treated rat aortic smooth muscle cells. / Wong, Kar Lok; Lin, Jia Wei; Liu, Ju Chi; Yang, Hung Yu; Kao, Pei Feng; Chen, Cheng Hsien; Loh, Shih Hurng; Chiu, Wen Ta; Cheng, Tzu-Hurng; Lin, Jaung Geng; Hong, Hong Jye.

於: Pharmacology, 卷 76, 編號 4, 04.2006, p. 163-169.

研究成果: 雜誌貢獻文章

Wong, Kar Lok ; Lin, Jia Wei ; Liu, Ju Chi ; Yang, Hung Yu ; Kao, Pei Feng ; Chen, Cheng Hsien ; Loh, Shih Hurng ; Chiu, Wen Ta ; Cheng, Tzu-Hurng ; Lin, Jaung Geng ; Hong, Hong Jye. / Antiproliferative effect of isosteviol on angiotensin-II-treated rat aortic smooth muscle cells. 於: Pharmacology. 2006 ; 卷 76, 編號 4. 頁 163-169.
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abstract = "Isosteviol is a derivative of stevioside, a constituent of Stevia rebaudiana, which is commonly used as a noncaloric sugar substitute in Japan and Brazil. The aims of this study were to examine whether isosteviol alters angiotensin-II-induced cell proliferation in rat aortic smooth muscle cells. Cultured rat aortic smooth muscle cells were preincubated with isosteviol, then stimulated with angiotensin II, after which [3H]thymidine incorporation and endothelin-1 secretion were examined. Isosteviol (1-100 μmol/l) inhibits angiotensin-II-induced DNA synthesis and endothelin-1 secretion. Measurements of 2′7′-dichlorofluorescin diacetate, a redox-sensitive fluorescent dye, showed an isosteviol-mediated inhibition of intracellular reactive oxygen species generated by the effects of angiotensin II. The inductive properties of angiotensin II on extracellular signal-regulated kinase (ERK) phosphorylation were found reversed with isosteviol and antioxidants such as N-acetylcysteine. In summary, we speculate that isosteviol inhibits angiotensin-II-induced cell proliferation and endothelin-1 secretion via attenuation of reactive oxygen species generation. Thus, this study provides important insights that may contribute to the effects of isosteviol on the cardiovascular system.",
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AU - Yang, Hung Yu

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AU - Chen, Cheng Hsien

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AU - Chiu, Wen Ta

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