32 引文 斯高帕斯(Scopus)

摘要

Isosteviol is a derivative of stevioside, a constituent of Stevia rebaudiana, which is commonly used as a noncaloric sugar substitute in Japan and Brazil. The aims of this study were to examine whether isosteviol alters angiotensin-II-induced cell proliferation in rat aortic smooth muscle cells. Cultured rat aortic smooth muscle cells were preincubated with isosteviol, then stimulated with angiotensin II, after which [3H]thymidine incorporation and endothelin-1 secretion were examined. Isosteviol (1-100 μmol/l) inhibits angiotensin-II-induced DNA synthesis and endothelin-1 secretion. Measurements of 2′7′-dichlorofluorescin diacetate, a redox-sensitive fluorescent dye, showed an isosteviol-mediated inhibition of intracellular reactive oxygen species generated by the effects of angiotensin II. The inductive properties of angiotensin II on extracellular signal-regulated kinase (ERK) phosphorylation were found reversed with isosteviol and antioxidants such as N-acetylcysteine. In summary, we speculate that isosteviol inhibits angiotensin-II-induced cell proliferation and endothelin-1 secretion via attenuation of reactive oxygen species generation. Thus, this study provides important insights that may contribute to the effects of isosteviol on the cardiovascular system.
原文英語
頁(從 - 到)163-169
頁數7
期刊Pharmacology
76
發行號4
DOIs
出版狀態已發佈 - 四月 2006

ASJC Scopus subject areas

  • Pharmacology

指紋 深入研究「Antiproliferative effect of isosteviol on angiotensin-II-treated rat aortic smooth muscle cells」主題。共同形成了獨特的指紋。

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