Antioxidant N-acetylcysteine blocks nerve growth factor-induced H 2O2/ERK signaling in PC12 cells

Liang-Yo Yang, Wun Chang Ko, Chun-Mao Lin, Jia-Wei Lin, Jen Chine Wu, Chien Ju Lin, Huey Hwa Cheng, Chwen-Ming Shih

研究成果: 雜誌貢獻文章

11 引文 斯高帕斯(Scopus)

摘要

We investigated whether H2O2, superoxide, and ERK participate in nerve growth factor (NGF)-induced signaling cascades and whether antioxidant N-acetylcysteine (NAC) regulates these NGF-induced responses. PC12 cells were cultured in medium containing NGF or vehicle with or without NAC pretreatment, and the intracellular H2O2 and superoxide levels and the amount of phosphorylated ERK were evaluated by flow cytometry and Western blotting, respectively. We found that NGF increased intracellular H2O2 concentration and activated ERK but failed to affect intracellular superoxide level. Moreover, NAC counteracted these NGF-induced responses. These findings demonstrate that NAC blocks the NGF-induced H 2O2/ERK signaling in PC12 cells.
原文英語
頁(從 - 到)325-337
頁數13
期刊Annals of the New York Academy of Sciences
1042
DOIs
出版狀態已發佈 - 2005

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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