Antioxidant effect of hydralazine on retinal pigment epithelial cells and its potential use in the therapy of age-related macular degeneration

Yu Wen Cheng, George C Y Chiou

研究成果: 雜誌貢獻文章同行評審

2 引文 斯高帕斯(Scopus)

摘要

Aim: To investigate the antioxidant effect of hydralazine under hypoxia-induced damage on retinal pigment epithelial (ARPE-19) cells and the role of reactive oxygen species (ROS) in this effect. • Methods: Human retinal pigment epithelial (hRPE) cells were used to investigate the effect of hydralazine on oxidative stress, including tert-butyl hydroxyperoxide (t-BHP), H2 O2, sodium azide (NaN3), and hypoxia induced cell damage. Cell viability was determined by MTT assay. • Results: When ARPE-19 cells were treated with oxidative stress induced by ROS, hydralazine showed concentration-dependent protection against t-BHP, H2 O2 and hypoxia induced cell damage but not NaN3. Nitric oxide (NO) was not involved in this effect. • Conclusion: Hydralazine showed antioxidant potential against oxidative stress induced damage in ARPE-19 cells. These effects might be caused through scavenger of ROS. Thus, hydralazine could be used for the treatment of age-related macular degeneration (AMD).
原文英語
頁(從 - 到)1059-1064
頁數6
期刊International Journal of Ophthalmology
8
發行號6
出版狀態已發佈 - 六月 25 2008
對外發佈Yes

Keywords

  • Age-related macular degeneration (AMD)
  • Reactive oxygen species (ROS)
  • Tea-butyl hydroxyperoxide (t-BHP)

ASJC Scopus subject areas

  • Ophthalmology

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