Antioxidant capability of polysaccharides fractionated from submerge-cultured Agaricus blazei mycelia

Yaw Bee Ker, Kuan Chou Chen, Charng Cherng Chyau, Chin Chu Chen, Jia Hsin Guo, Chiu Lan Hsieh, Hui Er Wang, Chiung Chi Peng, Chi Huang Chang, Robert Y. Peng

研究成果: 雜誌貢獻文章同行評審

63 引文 斯高帕斯(Scopus)

摘要

Five polysaccharide fractions (AB-1, AB-2, AB-3, AB-4, and AB-5) were obtained after a systemic solvent extraction and precipitation of Agaricus blazei mycelia with yields of 5.20, 9.03, 2.84, 17.47, and 0.44%, respectively. Among which, AB-1 and AB-3 demonstrated great DPPH radical scavenging ability around 85.0 and 72.0%, respectively, at a concentration of 5 mg/mL. The ferrous ion chelating powers were even more excellent at a concentration of 1 mg/mL, reaching almost over 99.65% for fractions AB-2, AB-3, and AB-4 as compared to the reference control of citric acid (35%); at a dosage of 5 mg/mL, fraction AB-1 even showed 105% in efficiency. In terms of the absolute chelating power (ACP), the mole numbers of ferrous (Fe2+) ions chelated were inversely related with the mean molecular mass of the polysaccharides in each fraction. In addition, gel permeation chromatography analysis showed that the more potent fractions were residing in those fractions with lower molecular masses, such as fractions AB-1 (757 kDa), AB-2 (887 kDa), and AB-4 (631 kDa) etc., and surprisingly, the free radical scavenging capability was also not closely correlated with the mean molecular masses, alternately being well-associated with the solubility of polysaccharides.

原文英語
頁(從 - 到)7052-7058
頁數7
期刊Journal of Agricultural and Food Chemistry
53
發行號18
DOIs
出版狀態已發佈 - 九月 7 2005

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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