Antioxidant, antinociceptive, and anti-inflammatory activities from actinidia callosa var. callosa in vitro and in vivo

Jung Chun Liao, Jeng Shyan Deng, Ying Chih Lin, Chao Ying Lee, Min Min Lee, Wen Chi Hou, Shyh Shyun Huang, Guan Jhong Huang

研究成果: 雜誌貢獻文章同行評審

10 引文 斯高帕斯(Scopus)

摘要

Actinidia callosa var. callosa has been widely used to treat antipyretic, analgesic, anti-inflammation, abdominal pain, and fever in Taiwan. The aim of this study was to evaluate the antioxidant, antinociceptive, and anti-inflammatory lipopolysaccharide-(LPS-)induced nitric oxide (NO) production in RAW264.7 macrophages and pawedema induced by -carrageenan activities of the methanol extract from A. callosa. In HPLC analysis, the fingerprint chromatogram of ethyl-acetate fraction of A. callosa (EAAC) was established. EAAC showed the highest TEAC and DPPH radical scavenging activities, respectively. We evaluated that EAAC and the reference compound of catechin and caffeic acid decreased the LPS-induced NO production in RAW264.7 cells. Treatment of male ICR mice with EAAC significantly inhibited the numbers of acetic acid-induced writhing response and the formalin-induced pain in the late phase. Administration of EAAC showed a concentration-dependent inhibition on paw edema development after Carr treatment in mice. Anti-inflammatory mechanisms of EAAC might be correlated to the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and heme oxygenase-1 (HO-1) in vitro and in vivo. Overall, the results showed that EAAC demonstrated antioxidant, antinociceptive, and anti-inflammatory activity, which supports previous claims of the traditional use for inflammation and pain.

原文英語
文章編號129152
期刊Evidence-based Complementary and Alternative Medicine
2012
DOIs
出版狀態已發佈 - 2012

ASJC Scopus subject areas

  • Complementary and alternative medicine

指紋 深入研究「Antioxidant, antinociceptive, and anti-inflammatory activities from actinidia callosa var. callosa in vitro and in vivo」主題。共同形成了獨特的指紋。

引用此