摘要
| 原文 | 英語 |
|---|---|
| 頁（從 - 到） | 195-202 |
| 頁數 | 8 |
| 期刊 | Food Chemistry |
| 卷 | 147 |
| DOIs | |
| 出版狀態 | 已發佈 - 2014 |
指紋
ASJC Scopus subject areas
- Food Science
- Analytical Chemistry
引用此文
Antioxidant and antiglycation activities of the synthesised dipeptide, Asn-Trp, derived from computer-aided simulation of yam dioscorin hydrolysis and its analogue, Gln-Trp. / Han, Chuan Hsiao; Lin, Yin Shiou; Lin, Shyr Yi; Hou, Wen Chi.
於: Food Chemistry, 卷 147, 2014, p. 195-202.研究成果: 雜誌貢獻 › 文章
}
TY - JOUR
T1 - Antioxidant and antiglycation activities of the synthesised dipeptide, Asn-Trp, derived from computer-aided simulation of yam dioscorin hydrolysis and its analogue, Gln-Trp
AU - Han, Chuan Hsiao
AU - Lin, Yin Shiou
AU - Lin, Shyr Yi
AU - Hou, Wen Chi
PY - 2014
Y1 - 2014
N2 - Previous studies have shown that the Trp residue contributes to a high oxygen radical absorbance capacity (ORAC). Therefore, in this study, a Trp-containing dipeptide derived from a computer-aided simulation of pepsin hydrolysis of the yam tuber, dioscorin - namely, Asn-Trp (NW), and its analogue, Gln-Trp (QW) - were synthesised to compare their antioxidant and antiglycation activities with carnosine, homocarnosine, or glutathione (GSH). The antioxidant assays included hydroxyl radical-scavenging activity, anti-AAPH-induced hemolysis, and ORAC activity. NW had a significantly higher antioxidant activity than had QW and performed much better than carnosine, homocarnosine, or GSH. Using bovine serum albumin (BSA)/galactose or BSA/glucose as experimental models, NW had better antiglycation effects than had QW, as detected by an anti-Nε-(carboxymethyl)lysine (anti-CML) antibody. Moreover, NW and QW (50-200 μM) showed protection against methylglyoxal-induced cell deaths in human umbilical vein endothelial cells. These results suggest that NW, derived from computer-aided simulation of dioscorin hydrolysis, exhibits antioxidant and antiglycation activities, which thus shows the benefits of the yam tuber as an antioxidant-rich food.
AB - Previous studies have shown that the Trp residue contributes to a high oxygen radical absorbance capacity (ORAC). Therefore, in this study, a Trp-containing dipeptide derived from a computer-aided simulation of pepsin hydrolysis of the yam tuber, dioscorin - namely, Asn-Trp (NW), and its analogue, Gln-Trp (QW) - were synthesised to compare their antioxidant and antiglycation activities with carnosine, homocarnosine, or glutathione (GSH). The antioxidant assays included hydroxyl radical-scavenging activity, anti-AAPH-induced hemolysis, and ORAC activity. NW had a significantly higher antioxidant activity than had QW and performed much better than carnosine, homocarnosine, or GSH. Using bovine serum albumin (BSA)/galactose or BSA/glucose as experimental models, NW had better antiglycation effects than had QW, as detected by an anti-Nε-(carboxymethyl)lysine (anti-CML) antibody. Moreover, NW and QW (50-200 μM) showed protection against methylglyoxal-induced cell deaths in human umbilical vein endothelial cells. These results suggest that NW, derived from computer-aided simulation of dioscorin hydrolysis, exhibits antioxidant and antiglycation activities, which thus shows the benefits of the yam tuber as an antioxidant-rich food.
KW - Antioxidant activity
KW - Asn-Trp
KW - Hemolysis
KW - N-(carboxymethyl) lysine (CML)
KW - Oxygen radical absorbance capacity (ORAC)
KW - Yam tuber dioscorin
UR - http://www.scopus.com/inward/record.url?scp=84885540735&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84885540735&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2013.09.109
DO - 10.1016/j.foodchem.2013.09.109
M3 - Article
C2 - 24206705
AN - SCOPUS:84885540735
VL - 147
SP - 195
EP - 202
JO - Food Chemistry
JF - Food Chemistry
SN - 0308-8146
ER -