Antimicrobial resistance mechanisms and virulence of colistin-and carbapenem-resistant acinetobacter baumannii isolated from a teaching hospital in taiwan

Noor Andryan Ilsan, Yuarn Jang Lee, Shu Chen Kuo, I. Hui Lee, Tzu Wen Huang

研究成果: 雜誌貢獻文章同行評審

1 引文 斯高帕斯(Scopus)

摘要

Acinetobacter baumannii, a Gram-negative bacterium, is an important nosocomial pathogen. Colistin-resistant A. baumannii is becoming a new concern, since colistin is one of the last-line antibiotics for infections by carbapenem-resistant A. baumannii. From 452 carbapenem-resistant isolates collected in a teaching hospital in Taipei, Taiwan, we identified seven that were resistant to colistin. Carbapenem resistance in these isolates is attributed to the presence of carbapenemase gene blaOXA-23 in their genomes. Colistin resistance is presumably conferred by mutations in the sensor kinase domain of PmrB found in these isolates, which are known to result in modification of colistin target lipid A via the PmrB–PmrA–PmrC signal transduction pathway. Overexpression of pmrC, eptA, and naxD was observed in all seven isolates. Colistin resistance mediated by pmrB mutations has never been reported in Taiwan. One of the seven isolates contained three mutations in lpxD and exhibited an altered lipopolysaccharide profile, which may contribute to its colistin resistance. No significant difference in growth rates was observed between the isolates and the reference strain, suggesting no fitness cost of colistin resistance. Biofilm formation abilities of the isolates were lower than that of the reference. Interestingly, one of the isolates was heteroresistant to colistin. Four of the isolates were significantly more virulent to wax moth larvae than the reference.
原文英語
文章編號1295
期刊Microorganisms
9
發行號6
DOIs
出版狀態已發佈 - 6月 2021

ASJC Scopus subject areas

  • 微生物學
  • 微生物學(醫學)
  • 病毒學

指紋

深入研究「Antimicrobial resistance mechanisms and virulence of colistin-and carbapenem-resistant acinetobacter baumannii isolated from a teaching hospital in taiwan」主題。共同形成了獨特的指紋。

引用此