Antifibrotic effect of bletilla striata polysaccharide-resveratrol-impregnated dual-layer carboxymethyl cellulose-based sponge for the prevention of epidural fibrosis after laminectomy

Hsuan Yu Chen, Tzu Chieh Lin, Chih Yung Chiang, Shiuan Li Wey, Feng Huei Lin, Kai Chiang Yang, Chih Hao Chang, Ming Hsiao Hu

研究成果: 雜誌貢獻文章同行評審

摘要

The application of antifibrotic materials can alleviate epidural fibrosis by restricting excessive fibroblast proliferation and mitigating scar tissue formation. Here, a biodegradable car-boxymethyl cellulose (CMC)-Bletilla striata polysaccharide (BSP)-resveratrol (RES) sponge was fabricated to inhibit scar tissue formation post laminectomy surgery. Fibroblasts NIH/3T3, myoblasts C2C12, neural cells PC-12, and Schwann cells RSC96 were used to evaluate the in vitro cytocompati-bility. Laminectomies on 10 Sprague–Dawley rats with/without the application of the CMC-BSP-RES sponge were performed. The severity of adhesion between the dura mater and formed scar tissue was qualitatively scored. All cell lines exhibited good viability with no significant difference in cytotoxicity when cultured with variable extractions of the CMC-BSP-RES sponge. S100a4 and P4hb expressions were downregulated in NIH/3T3 cultured in the CMC-BSP-RES sponge, implying that this sponge potentially inhibits fibroblast activity. No post-operative shrinkage or dura mater expan-sion along the surgical site was detected. The peel-off tests revealed that the tenacity of adhesion de-creased. Histopathological examinations verified that the average number of fibroblasts in the CMC-BSP-RES group considerably decreased. The CMC-BSP-RES sponge is a biocompatible and effective material for alleviating post-operative epidural fibrosis and mitigating fibroblast expression following laminectomy.
原文英語
文章編號2129
期刊Polymers
13
發行號13
DOIs
出版狀態已發佈 - 七月 2021

ASJC Scopus subject areas

  • 化學 (全部)
  • 聚合物和塑料

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