摘要
| 原文 | 英語 |
|---|---|
| 頁（從 - 到） | 236-243 |
| 頁數 | 8 |
| 期刊 | Reproductive Sciences |
| 卷 | 21 |
| 發行號 | 2 |
| DOIs | |
| 出版狀態 | 已發佈 - 2014 |
指紋
ASJC Scopus subject areas
- Obstetrics and Gynaecology
引用此文
Antiapoptotic agent sphingosine-1-phosphate protects vitrified murine ovarian grafts. / Tsai, Yung Chieh; Tzeng, Chii Ruey; Wang, Chia-Woei; Hsu, Ming I.; Tan, Shun Jen; Chen, Chi Huang.
於: Reproductive Sciences, 卷 21, 編號 2, 2014, p. 236-243.研究成果: 雜誌貢獻 › 文章
}
TY - JOUR
T1 - Antiapoptotic agent sphingosine-1-phosphate protects vitrified murine ovarian grafts
AU - Tsai, Yung Chieh
AU - Tzeng, Chii Ruey
AU - Wang, Chia-Woei
AU - Hsu, Ming I.
AU - Tan, Shun Jen
AU - Chen, Chi Huang
PY - 2014
Y1 - 2014
N2 - Significant follicle loss from frozen ovarian grafts is unavoidable. The authors evaluated the protective effects of the antiapoptotic agent sphingosine-1-phosphate (S1P) on vitrified ovarian grafts. Three-week-old sexually immature female FVB mice were divided into 4 groups, fresh, control without S1P, 0.5 mmol/L S1P, and 2 mmol/L S1P. The ovaries were pretreated with S1P for 1 hour and then cryopreserved by modified vitrification. The frozen-thawed ovaries were autotransplanted under the back muscles of mice for 10 days. Expression of apoptosis-related genes encoding caspase 3 and c-Myc was analyzed in the vitrified ovaries and 10 days after transplantation using real-time quantitative polymerase chain reaction. To quantify the ovarian reserve, anti-Müllerian hormone (AMH) levels and follicles were measured in the 10-day vitrified ovarian grafts. Caspase 3 and c-Myc messenger RNA did not differ significantly in the 4 groups after vitrification but was significantly upregulated in the control group after transplantation. The AMH levels and primordial follicle pool were significantly higher in the S1P-treated groups than in the control group but lower than that in the fresh group. The S1P protects vitrified ovarian grafts from ischemic reperfusion injury rather than from vitrification-associated process.
AB - Significant follicle loss from frozen ovarian grafts is unavoidable. The authors evaluated the protective effects of the antiapoptotic agent sphingosine-1-phosphate (S1P) on vitrified ovarian grafts. Three-week-old sexually immature female FVB mice were divided into 4 groups, fresh, control without S1P, 0.5 mmol/L S1P, and 2 mmol/L S1P. The ovaries were pretreated with S1P for 1 hour and then cryopreserved by modified vitrification. The frozen-thawed ovaries were autotransplanted under the back muscles of mice for 10 days. Expression of apoptosis-related genes encoding caspase 3 and c-Myc was analyzed in the vitrified ovaries and 10 days after transplantation using real-time quantitative polymerase chain reaction. To quantify the ovarian reserve, anti-Müllerian hormone (AMH) levels and follicles were measured in the 10-day vitrified ovarian grafts. Caspase 3 and c-Myc messenger RNA did not differ significantly in the 4 groups after vitrification but was significantly upregulated in the control group after transplantation. The AMH levels and primordial follicle pool were significantly higher in the S1P-treated groups than in the control group but lower than that in the fresh group. The S1P protects vitrified ovarian grafts from ischemic reperfusion injury rather than from vitrification-associated process.
KW - anti-Müllerian hormone
KW - apoptosis
KW - sphingosine-1-phosphate
KW - vitrification
UR - http://www.scopus.com/inward/record.url?scp=84901766835&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901766835&partnerID=8YFLogxK
U2 - 10.1177/1933719113493515
DO - 10.1177/1933719113493515
M3 - Article
C2 - 23793475
AN - SCOPUS:84901766835
VL - 21
SP - 236
EP - 243
JO - Reproductive Sciences
JF - Reproductive Sciences
SN - 1933-7191
IS - 2
ER -