Antiangiogenic activity of indole-3-carbinol in endothelial cells stimulated with activated macrophages

Mei Ling Wang, Chun-Kuang Shih, H. P. Chang, Yue-Hwa Chen

研究成果: 雜誌貢獻文章

15 引文 (Scopus)

摘要

The effect of indole-3-carbinol (I3C), a major indolic metabolite in cruciferous vegetables, on lipopolysaccharide (LPS)-activated macrophage-induced tube formation and its associated factors in endothelial EA hy926 cells was investigated. LPS significantly enhanced the capillary-like structure of endothelial cells (ECs) co-cultured with macrophages, but no such effect was observed in single-cultured ECs. I3C, on the other hand, suppressed such enhancement in concert with decreased secretions of vascular endothelial growth factor (VEGF), nitric oxide (NO), interleukin-6 (IL-6), and matrix metalloproteinases (MMPs). The results obtained from cultivating ECs with conditioned medium (CM) collected from macrophages suggested that both ECs and macrophages were inactivated by I3C. These results indicate that I3C from cruciferous vegetables may possess potential roles in preventing inflammation-associated angiogenic diseases.
原文英語
頁(從 - 到)811-820
頁數10
期刊Food Chemistry
134
發行號2
DOIs
出版狀態已發佈 - 九月 15 2012

指紋

Macrophages
Endothelial cells
indoles
endothelial cells
macrophages
Endothelial Cells
Vegetables
lipopolysaccharides
Lipopolysaccharides
vegetables
vascular endothelial growth factors
metalloproteinases
Conditioned Culture Medium
Metabolites
Matrix Metalloproteinases
interleukin-6
cultured cells
Vascular Endothelial Growth Factor A
nitric oxide
Cultured Cells

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

引用此文

Antiangiogenic activity of indole-3-carbinol in endothelial cells stimulated with activated macrophages. / Wang, Mei Ling; Shih, Chun-Kuang; Chang, H. P.; Chen, Yue-Hwa.

於: Food Chemistry, 卷 134, 編號 2, 15.09.2012, p. 811-820.

研究成果: 雜誌貢獻文章

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title = "Antiangiogenic activity of indole-3-carbinol in endothelial cells stimulated with activated macrophages",
abstract = "The effect of indole-3-carbinol (I3C), a major indolic metabolite in cruciferous vegetables, on lipopolysaccharide (LPS)-activated macrophage-induced tube formation and its associated factors in endothelial EA hy926 cells was investigated. LPS significantly enhanced the capillary-like structure of endothelial cells (ECs) co-cultured with macrophages, but no such effect was observed in single-cultured ECs. I3C, on the other hand, suppressed such enhancement in concert with decreased secretions of vascular endothelial growth factor (VEGF), nitric oxide (NO), interleukin-6 (IL-6), and matrix metalloproteinases (MMPs). The results obtained from cultivating ECs with conditioned medium (CM) collected from macrophages suggested that both ECs and macrophages were inactivated by I3C. These results indicate that I3C from cruciferous vegetables may possess potential roles in preventing inflammation-associated angiogenic diseases.",
keywords = "Activated macrophages, Angiogenesis, Co-culture, Cruciferous vegetables, Indole-3-carbinol, Tube formation",
author = "Wang, {Mei Ling} and Chun-Kuang Shih and Chang, {H. P.} and Yue-Hwa Chen",
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AU - Wang, Mei Ling

AU - Shih, Chun-Kuang

AU - Chang, H. P.

AU - Chen, Yue-Hwa

PY - 2012/9/15

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N2 - The effect of indole-3-carbinol (I3C), a major indolic metabolite in cruciferous vegetables, on lipopolysaccharide (LPS)-activated macrophage-induced tube formation and its associated factors in endothelial EA hy926 cells was investigated. LPS significantly enhanced the capillary-like structure of endothelial cells (ECs) co-cultured with macrophages, but no such effect was observed in single-cultured ECs. I3C, on the other hand, suppressed such enhancement in concert with decreased secretions of vascular endothelial growth factor (VEGF), nitric oxide (NO), interleukin-6 (IL-6), and matrix metalloproteinases (MMPs). The results obtained from cultivating ECs with conditioned medium (CM) collected from macrophages suggested that both ECs and macrophages were inactivated by I3C. These results indicate that I3C from cruciferous vegetables may possess potential roles in preventing inflammation-associated angiogenic diseases.

AB - The effect of indole-3-carbinol (I3C), a major indolic metabolite in cruciferous vegetables, on lipopolysaccharide (LPS)-activated macrophage-induced tube formation and its associated factors in endothelial EA hy926 cells was investigated. LPS significantly enhanced the capillary-like structure of endothelial cells (ECs) co-cultured with macrophages, but no such effect was observed in single-cultured ECs. I3C, on the other hand, suppressed such enhancement in concert with decreased secretions of vascular endothelial growth factor (VEGF), nitric oxide (NO), interleukin-6 (IL-6), and matrix metalloproteinases (MMPs). The results obtained from cultivating ECs with conditioned medium (CM) collected from macrophages suggested that both ECs and macrophages were inactivated by I3C. These results indicate that I3C from cruciferous vegetables may possess potential roles in preventing inflammation-associated angiogenic diseases.

KW - Activated macrophages

KW - Angiogenesis

KW - Co-culture

KW - Cruciferous vegetables

KW - Indole-3-carbinol

KW - Tube formation

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