Anti-Proliferative Effects of Evodiamine on Human Breast Cancer Cells

Kai Lee Wang, Shih Min Hsia, Jiun Yih Yeh, Shao Chi Cheng, Paulus S. Wang, Shyi Wu Wang

研究成果: 雜誌貢獻文章

31 引文 斯高帕斯(Scopus)

摘要

Endocrine sensitivity, assessed by the expression of estrogen receptor (ER), has long been the predict factor to guide therapeutic decisions. Tamoxifen has been the most successful hormonal treatment in endocrine-sensitive breast cancer. However, in estrogen-insensitive cancer tamoxifen showed less effectiveness than in estrogen-sensitive cancer. It is interesting to develop new drugs against both hormone-sensitive and insensitive tumor. In this present study we examined anticancer effects of evodiamine extracted from the Chinese herb, Evodiae fructus, in estrogen-dependent and -independent human breast cancer cells, MCF-7 and MDA-MB-231 cells, respectively. Evodiamine inhibited the proliferation of MCF-7 and MDA-MB-231 cells in a concentration-dependent manner with concentration of 1×10-6 and 1×10-5 M. Evodiamine also induced apoptosis via up-regulation of caspase 7 activation, PARP cleavage (Bik and Bax expression). The expression of ER α and β in protein and mRNA levels was down-regulated by evodiamine according to data from immunoblotting and RT-PCR analysis. Overall, our results indicate that evodiamine mediates degradation of ER and induces caspase-dependent pathway leading to inhibit proliferation of breast cancer cell lines. It suggests that evodiamine may in part mediate through ER-inhibitory pathway to inhibit breast cancer cell proliferation.

原文英語
文章編號e67297
期刊PLoS One
8
發行號6
DOIs
出版狀態已發佈 - 六月 26 2013

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

指紋 深入研究「Anti-Proliferative Effects of Evodiamine on Human Breast Cancer Cells」主題。共同形成了獨特的指紋。

  • 引用此

    Wang, K. L., Hsia, S. M., Yeh, J. Y., Cheng, S. C., Wang, P. S., & Wang, S. W. (2013). Anti-Proliferative Effects of Evodiamine on Human Breast Cancer Cells. PLoS One, 8(6), [e67297]. https://doi.org/10.1371/journal.pone.0067297