Anti-inflammatory effects of peptides from a marine algicolous fungus acremonium sp. Ntu492 in bv-2 microglial cells

George Hsiao, Shih Wei Wang, Yin Ru Chiang, Wei Chiung Chi, Yueh Hsiung Kuo, Do Anh Phong, Chia Yu Chen, Tzong Huei Lee

研究成果: 雜誌貢獻文章同行評審

3 引文 斯高帕斯(Scopus)

摘要

Located in tropical and subtropical region, Taiwan is an island with high algal species diversity. In this study, a number of fungal strains were isolated from marine macroalgae collected from northeastern intertidal zone of Taiwan. Preliminary anti-inflammatory screening has shown that the methanolic extracts of solid fermented products of the red alga Mastophora rosea-derived fungal strain Acremonium sp. NTU492 exhibited significant bioactivity. In an attempt to disclose the active principles from this fungal strain, a series of separation and purification was thus undertaken, which has led to the isolation and characterization of seven compounds including four new peptides, namely acrepeptins A‒D (1e4), along with previously reported destruxin B (5), guangomide A (6), and guangomide B (7). Their structures were elucidated by spectroscopic analysis and compared with literatures. Of these, acrepeptins A (1) and C (3) showed markedly inhibitory activities on nitric oxide production in lipopolysaccharide-activated microglial BV-2 cells with IC50 values of 12.0 ± 2.3 and 10.6 ± 4.0 mM, respectively. Furthermore, acrepeptins A (1) and C (3) significantly attenuated the expression of inducible nitric oxide synthase in a concentration-dependent manner (5e40 mM).
原文英語
文章編號9
頁(從 - 到)283-291
頁數9
期刊Journal of Food and Drug Analysis
28
發行號2
DOIs
出版狀態已發佈 - 2020

ASJC Scopus subject areas

  • 食品科學
  • 藥理

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