Anti-inflammatory Effects of Gossypol on Human Lymphocytic Jurkat Cells via Regulation of MAPK Signaling and Cell Cycle

Chien Wei Chen, Sindy Hu, Ke Hung Tsui, Guey Shyang Hwang, Szu Tah Chen, Tswen Kei Tang, Hao Tsai Cheng, Ju Wen Yu, Hsiao Chiu Wang, Horng Heng Juang, Paulus S. Wang, Shyi Wu Wang

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9 引文 斯高帕斯(Scopus)

摘要

Gossypol, a natural polyphenolic compound extracted from cottonseed oil, has been reported to possess pharmacological properties via modulation cell cycle and immune signaling pathway. However, whether gossypol has anti-inflammatory effects against phytohemagglutinin (PHA)-induced cytokine secretion in T lymphocytes through similar mechanism remains unclear. Using the T lymphocytes Jurkat cell line, we found that PHA exposure caused dramatic increase in interleukin-2 (IL-2) mRNA expression as well as IL-2 secretion. All of these PHA-stimulated reactions were attenuated in a dose-dependent manner by being pretreated with gossypol. However, gossypol did not show any in vitro cytotoxic effect at doses of 5–20 μM. As a possible mechanism underlying gossypol action, such as pronounced suppression IL-2 release, robust decreased PHA-induced phosphorylation of p38 and c-Jun N-terminal kinase expressions was found with gossypol pretreatment, but not significant phosphorylation of extracellular signal-regulated kinase expression. Furthermore, gossypol could suppress the Jurkat cells’ growth, which was associated with increased percentage of G1/S phase and decreased fraction of G2 phase in flow cytometry test. We conclude that gossypol exerts anti-inflammatory effects probably through partial attenuation of mitogen-activated protein kinase (phosphorylated JNK and p38) signaling and cell cycle arrest in Jurkat cells.
原文英語
頁(從 - 到)2265-2274
頁數10
期刊Inflammation
41
發行號6
DOIs
出版狀態已發佈 - 12月 2018
對外發佈

ASJC Scopus subject areas

  • 免疫學和過敏
  • 免疫學

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