Anthocyanins induce the activation of phase II enzymes through the antioxidant response element pathway against oxidative stress-induced apoptosis

Ping Hsiao Shih, Chi T. Yeh, Gow Chin Yen

研究成果: 雜誌貢獻文章

177 引文 (Scopus)

摘要

Reactive oxygen species (ROS)-induced cell damage is inevitable and severe and is involved in numerous diseases, including cancer. Reducing oxidative stress is one of the strategies of chemoprevention. Anthocyanins are naturally occurring flavonoids that show multiple benefits. We first pointed out the effects of anthocyanins in the contributions to activation of phase II antioxidant and detoxifying enzymes, chemopreventive potency, and involved transcriptional regulation. Our results obtained in rat liver Clone 9 cells showed that treatment of anthocyanins leads to positive effects on elevating the antioxidant capacity, including activated expression of glutathione-related enzymes (glutathione reductase, glutathione peroxidase, and glutathione S-transferase) and recruited GSH content. In addition, the activity of NAD(P)H: quinone oxidoreductase (NQO1) was also promoted under the treatment of anthocyanin. This influential functions as the defense system against programmed cell death induced by H2O2. The capacity for induction of luciferase expression by anthocyanins in cells transfected with rat nqo1-promoter constructed plasmid was further investigated; we found that the molecular mechanism is related to the activation of antioxidant response element (ARE) upstream of genes that are involved in antioxidation and detoxification. Our data suggest that natural anthocyanins are recommended as chemopreventive phytochemicals and could stimulate the antioxidant system to resist oxidant-induced injury. And, more important, the promoting effect of anthocyanins on ARE-regulated phase II enzyme expression seems to be a critical point in modulating the defense system against oxidative stress.
原文英語
頁(從 - 到)9427-9435
頁數9
期刊Journal of Agricultural and Food Chemistry
55
發行號23
DOIs
出版狀態已發佈 - 十一月 14 2007
對外發佈Yes

指紋

Antioxidant Response Elements
Oxidative stress
Anthocyanins
response elements
anthocyanins
Oxidative Stress
oxidative stress
apoptosis
Chemical activation
Apoptosis
antioxidants
Enzymes
enzymes
Antioxidants
Rats
Detoxification
chemoprevention
NAD(P)H dehydrogenase (quinone)
Glutathione Reductase
rats

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

引用此文

Anthocyanins induce the activation of phase II enzymes through the antioxidant response element pathway against oxidative stress-induced apoptosis. / Shih, Ping Hsiao; Yeh, Chi T.; Yen, Gow Chin.

於: Journal of Agricultural and Food Chemistry, 卷 55, 編號 23, 14.11.2007, p. 9427-9435.

研究成果: 雜誌貢獻文章

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abstract = "Reactive oxygen species (ROS)-induced cell damage is inevitable and severe and is involved in numerous diseases, including cancer. Reducing oxidative stress is one of the strategies of chemoprevention. Anthocyanins are naturally occurring flavonoids that show multiple benefits. We first pointed out the effects of anthocyanins in the contributions to activation of phase II antioxidant and detoxifying enzymes, chemopreventive potency, and involved transcriptional regulation. Our results obtained in rat liver Clone 9 cells showed that treatment of anthocyanins leads to positive effects on elevating the antioxidant capacity, including activated expression of glutathione-related enzymes (glutathione reductase, glutathione peroxidase, and glutathione S-transferase) and recruited GSH content. In addition, the activity of NAD(P)H: quinone oxidoreductase (NQO1) was also promoted under the treatment of anthocyanin. This influential functions as the defense system against programmed cell death induced by H2O2. The capacity for induction of luciferase expression by anthocyanins in cells transfected with rat nqo1-promoter constructed plasmid was further investigated; we found that the molecular mechanism is related to the activation of antioxidant response element (ARE) upstream of genes that are involved in antioxidation and detoxification. Our data suggest that natural anthocyanins are recommended as chemopreventive phytochemicals and could stimulate the antioxidant system to resist oxidant-induced injury. And, more important, the promoting effect of anthocyanins on ARE-regulated phase II enzyme expression seems to be a critical point in modulating the defense system against oxidative stress.",
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