Annexin A2 on lung epithelial cell surface is recognized by severe acute respiratory syndrome-associated coronavirus spike domain 2 antibodies

Yi Ting Fang, Chiou Feng Lin, Pao Chi Liao, Yu Min Kuo, Shuying Wang, Trai Ming Yeh, Chi Chang K Shieh, Ih Jen Su, Huan Yao Lei, Yee Shin Lin

研究成果: 雜誌貢獻文章

10 引文 (Scopus)

摘要

Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection causes lung failure characterized by atypical pneumonia. We previously showed that antibodies against SARS-CoV spike domain 2 (S2) in the patient sera can cross-react with human lung epithelial cells; however, the autoantigen is not yet identified. In this study, we performed proteomic studies and identified several candidate autoantigens recognized by SARS patient sera in human lung type II epithelial cell A549. Among the candidate proteins, annexin A2, which was identified by mass spectrometry analysis and had the highest score by Mascot data search, was further characterized and investigated for its role as an autoantigen. By confocal microscopic observation, SARS patient sera and anti-S2 antibodies were co-localized on A549 cells and both of them were co-localized with anti-annexin A2 antibodies. Anti-annexin A2 antibodies bound to purified S2 proteins, and anti-S2 bound to immunoprecipitated annexin A2 from A549 cell lysate in a dose-dependent manner. Furthermore, an increased surface expression and raft-structure distribution of annexin A2 was present in A549 cells after stimulation with SARS-induced cytokines interleukin-6 and interferon-γ. Cytokine stimulation increased the binding capability of anti-S2 antibodies to human lung epithelial cells. Together, the upregulated expression of annexin A2 by SARS-associated cytokines and the cross-reactivity of anti-SARS-CoV S2 antibodies to annexin A2 may have implications in SARS disease pathogenesis.

原文英語
頁(從 - 到)1000-1009
頁數10
期刊Molecular Immunology
47
發行號5
DOIs
出版狀態已發佈 - 二月 2010
對外發佈Yes

指紋

Annexin A2
Severe Acute Respiratory Syndrome
Coronavirus
Epithelial Cells
Lung
Antibodies
Autoantigens
Cytokines
Serum
Coronavirus Infections
Proteomics
Interferons
Interleukin-6
Mass Spectrometry
Pneumonia

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

引用此文

Annexin A2 on lung epithelial cell surface is recognized by severe acute respiratory syndrome-associated coronavirus spike domain 2 antibodies. / Fang, Yi Ting; Lin, Chiou Feng; Liao, Pao Chi; Kuo, Yu Min; Wang, Shuying; Yeh, Trai Ming; Shieh, Chi Chang K; Su, Ih Jen; Lei, Huan Yao; Lin, Yee Shin.

於: Molecular Immunology, 卷 47, 編號 5, 02.2010, p. 1000-1009.

研究成果: 雜誌貢獻文章

Fang, Yi Ting ; Lin, Chiou Feng ; Liao, Pao Chi ; Kuo, Yu Min ; Wang, Shuying ; Yeh, Trai Ming ; Shieh, Chi Chang K ; Su, Ih Jen ; Lei, Huan Yao ; Lin, Yee Shin. / Annexin A2 on lung epithelial cell surface is recognized by severe acute respiratory syndrome-associated coronavirus spike domain 2 antibodies. 於: Molecular Immunology. 2010 ; 卷 47, 編號 5. 頁 1000-1009.
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title = "Annexin A2 on lung epithelial cell surface is recognized by severe acute respiratory syndrome-associated coronavirus spike domain 2 antibodies",
abstract = "Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection causes lung failure characterized by atypical pneumonia. We previously showed that antibodies against SARS-CoV spike domain 2 (S2) in the patient sera can cross-react with human lung epithelial cells; however, the autoantigen is not yet identified. In this study, we performed proteomic studies and identified several candidate autoantigens recognized by SARS patient sera in human lung type II epithelial cell A549. Among the candidate proteins, annexin A2, which was identified by mass spectrometry analysis and had the highest score by Mascot data search, was further characterized and investigated for its role as an autoantigen. By confocal microscopic observation, SARS patient sera and anti-S2 antibodies were co-localized on A549 cells and both of them were co-localized with anti-annexin A2 antibodies. Anti-annexin A2 antibodies bound to purified S2 proteins, and anti-S2 bound to immunoprecipitated annexin A2 from A549 cell lysate in a dose-dependent manner. Furthermore, an increased surface expression and raft-structure distribution of annexin A2 was present in A549 cells after stimulation with SARS-induced cytokines interleukin-6 and interferon-γ. Cytokine stimulation increased the binding capability of anti-S2 antibodies to human lung epithelial cells. Together, the upregulated expression of annexin A2 by SARS-associated cytokines and the cross-reactivity of anti-SARS-CoV S2 antibodies to annexin A2 may have implications in SARS disease pathogenesis.",
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AU - Fang, Yi Ting

AU - Lin, Chiou Feng

AU - Liao, Pao Chi

AU - Kuo, Yu Min

AU - Wang, Shuying

AU - Yeh, Trai Ming

AU - Shieh, Chi Chang K

AU - Su, Ih Jen

AU - Lei, Huan Yao

AU - Lin, Yee Shin

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AB - Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection causes lung failure characterized by atypical pneumonia. We previously showed that antibodies against SARS-CoV spike domain 2 (S2) in the patient sera can cross-react with human lung epithelial cells; however, the autoantigen is not yet identified. In this study, we performed proteomic studies and identified several candidate autoantigens recognized by SARS patient sera in human lung type II epithelial cell A549. Among the candidate proteins, annexin A2, which was identified by mass spectrometry analysis and had the highest score by Mascot data search, was further characterized and investigated for its role as an autoantigen. By confocal microscopic observation, SARS patient sera and anti-S2 antibodies were co-localized on A549 cells and both of them were co-localized with anti-annexin A2 antibodies. Anti-annexin A2 antibodies bound to purified S2 proteins, and anti-S2 bound to immunoprecipitated annexin A2 from A549 cell lysate in a dose-dependent manner. Furthermore, an increased surface expression and raft-structure distribution of annexin A2 was present in A549 cells after stimulation with SARS-induced cytokines interleukin-6 and interferon-γ. Cytokine stimulation increased the binding capability of anti-S2 antibodies to human lung epithelial cells. Together, the upregulated expression of annexin A2 by SARS-associated cytokines and the cross-reactivity of anti-SARS-CoV S2 antibodies to annexin A2 may have implications in SARS disease pathogenesis.

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