Anisotropic diffusion deviates chicken embryo chorioallantoic membrane assay (CAM) to reflect inherent therapeutic behaviors

Chiung Chi Peng, Chiu Lan Hsieh, Li Yun Lin, Charng Cherng Chyau, Yu Ting Cheng, Kuan Chou Chen, Robert Y. Peng

研究成果: 雜誌貢獻文章同行評審

4 引文 斯高帕斯(Scopus)

摘要

Chorioallantoic membrane assay (CAM) has become a widely used tool for determination of anti-angiogenesis capability of many drugs including herbal extracts. Because varying results in same set of chicken embryos are often encountered, we developed the complex diffusion model that combined the Fick's second diffusion law, chemical-protein interaction (or binding) to explain the diffusion- or kinetic-limiting phenomena in egg white when performing CAM. In addition, we performed diffusion studies in egg white with Color Blue No. 1, Evans Blue, Color Red No. 40, and the aqueous extract of Psidium guajava budding leaves (PE) to support our model. Under same conditions, the diffusion coefficients of Blue No. 1, Evans Blue, Red No. 40, and PE were (2.0-2.8) × 10-9, (0.89-31) × 10-9, (2.8-12) × 10-9, and (7.0-21) × 10-9 m2 s-1, respectively, depending upon the distance diffused. Whilst at the interface of egg white and embryo (egg yolk), a site about 1 cm apart from the aeration sac, the percent concentration reached only 10.5, 3.0, 3.6, and 2.2% of the original applied medicine, respectively. We conclude that CAM could only serve as a preliminary screening tool for angiogenesis, because the anisotropic diffusion in egg white affects greatly the effective dosages of medicines tested.

原文英語
頁(從 - 到)29-34
頁數6
期刊Colloids and Surfaces B: Biointerfaces
70
發行號1
DOIs
出版狀態已發佈 - 四月 1 2009
對外發佈

ASJC Scopus subject areas

  • 生物技術
  • 膠體和表面化學
  • 物理與理論化學
  • 表面和介面

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