摘要
The effect of cyclical mechanical stretch on angiotensinogen gene expression was examined using a neonatal rat cardiocyte culture system. Cultured cardiocytes grown on a flexible membrane base were stretched by vacuum to 20% of maximum elongation, at 60 cycles/min. The angiotensinogen gene was activated 2 to 5 fold after stretch for 3 to 24 hr, as shown by quantitative reverse transcription polymerase chain reaction. The 5'-flanking region of the angiotensinogen promoter was activated after stretch for 24 hr. This gene expression could be completely suppressed by losartan, a specific antagonist of angiotensin II receptor. These results indicate that (1) cyclical mechanical stretching of cardiocytes is a good model for the study of cardiac hypertrophy-related gene expression; (2) cyclical stretch up-regulates expression of the angiotensinogen gene and (3) the increase in promoter activity may contribute to the induction of angiotensinogen mRNA by cyclical stretch.
原文 | 英語 |
---|---|
頁(從 - 到) | 241-248 |
頁數 | 8 |
期刊 | Biochemical and Biophysical Research Communications |
卷 | 211 |
發行號 | 1 |
DOIs | |
出版狀態 | 已發佈 - 1995 |
對外發佈 | 是 |
ASJC Scopus subject areas
- 生物化學
- 生物物理學
- 分子生物學
- 細胞生物學