Angiotensinogen gene expression is induced by cyclical mechanical stretch in cultured rat cardiomyocytes

K. G. Shyu, J. J. Chen, N. L. Shih, H. Chang, D. L. Wang, W. P. Lien, C. C. Liew

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48 引文 斯高帕斯(Scopus)


The effect of cyclical mechanical stretch on angiotensinogen gene expression was examined using a neonatal rat cardiocyte culture system. Cultured cardiocytes grown on a flexible membrane base were stretched by vacuum to 20% of maximum elongation, at 60 cycles/min. The angiotensinogen gene was activated 2 to 5 fold after stretch for 3 to 24 hr, as shown by quantitative reverse transcription polymerase chain reaction. The 5'-flanking region of the angiotensinogen promoter was activated after stretch for 24 hr. This gene expression could be completely suppressed by losartan, a specific antagonist of angiotensin II receptor. These results indicate that (1) cyclical mechanical stretching of cardiocytes is a good model for the study of cardiac hypertrophy-related gene expression; (2) cyclical stretch up-regulates expression of the angiotensinogen gene and (3) the increase in promoter activity may contribute to the induction of angiotensinogen mRNA by cyclical stretch.

頁(從 - 到)241-248
期刊Biochemical and Biophysical Research Communications
出版狀態已發佈 - 1995

ASJC Scopus subject areas

  • 生物化學
  • 生物物理學
  • 分子生物學
  • 細胞生物學


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