Angiotensin II stimulates hypoxia-inducible factor 1α accumulation in glomerular mesangial cells

Tso Hsiao Chen, Jin Fong Wang, Paul Chan, Horng Mo Lee

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23 引文 斯高帕斯(Scopus)

摘要

Hypoxia increases hypoxia-inducible factor 1α (HIF-1α) protein levels by inhibiting ubiquitination and degradation of HIF-1α, which regulates the transcription of many genes. Recent studies have revealed that many ligands can stimulate HIF-1α accumulation under nonhypoxic conditions. In this study, we show that angiotensin II (Ang II) increased HIF-1α protein levels in a time- and dose-dependent manner under normoxic conditions. Treatment of mesangial cells with Ang II (100 nM) increased production of reactive oxygen species (ROS). Ang II (100 nM) increased the phosphorylation of PDK-1 and Akt/PKB in glomerular mesangial cells. Ang II-stimulated HIF-1α accumulation was blocked by the phosphatidylinositol 3-kinase (PI-3K) inhibitors, Ly 294001, and wortmannin, suggesting that PI-3K was involved. Because increased ROS generation by Ang II may activate the PI-3K-PKB/Akt signaling pathway, these results suggest that Ang II may stimulate a ROS-dependent activation of the PI-3K-PKB/Akt pathway, which leads to HIF-1α accumulation.
原文英語
頁(從 - 到)286-293
頁數8
期刊Annals of the New York Academy of Sciences
1042
DOIs
出版狀態已發佈 - 2005

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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