Angiopoietins modulate endothelial adaptation, glomerular and podocyte hypertrophy after uninephrectomy

Wen Chih Chiang, Chun Fu Lai, Chi Ting Su, Wei Hao Peng, Ching Fang Wu, Fan Chi Chang, Yi Ting Chen, Shuei Liong Lin, Yung Ming Chen, Kwan Dun Wu, Kuo Shyan Lu, Tun Jun Tsai

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2 引文 斯高帕斯(Scopus)

摘要

Glomerular capillary remodeling is an essential process in the development of glomerular hypertrophy. Angiopoietins, which are important regulators in angiogenesis, plays a role in the development of glomerulus during embryogenesis. Here, we evaluated the influence of angiopoietin on glomerular components and hypertrophy after uninephrectomy in adult male BALB/c mice. The actions of angiopoietin 1 or 2 were systemically antagonized by the subcutaneous administration of antagonists. We observed that the angiopoietin system was activated after uninephrectomy, and that the blockade of angiopoietin 1 or 2 decreased the activation of the angiopoietin receptor - tyrosine kinase with Ig and EGF homology domains-2 - and attenuated the development of glomerular and podocyte hypertrophy. The increase in endothelial density staining (anti-CD31) following uninephrectomy was also reversed by angiopoietin 1 or 2 blockades. Glomerular basement thickness and foot process width were observed to decrease in the angiopoietin blockade groups. These changes were associated with the down regulation of the expression of genes for the glomerular matrix and basement membrane, including collagen type IV α1, collagen type IV α2, collagen type IV α5, and laminin α5. Thus, angiopoietin 1 or 2 may play an important role in the development of glomerular hypertrophy after uninephrectomy. A blockade of the angiopoietin system not only influenced the endothelium but also the podocyte, leading to diminished gene expression and morphological changes after uninephrectomy.

原文英語
文章編號e82592
期刊PLoS One
8
發行號12
DOIs
出版狀態已發佈 - 12月 18 2013
對外發佈

ASJC Scopus subject areas

  • 農業與生物科學 (全部)
  • 生物化學、遺傳與分子生物學 (全部)
  • 醫藥 (全部)

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