Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway

Tzu Shao Yeh, Cheng-Chen Hsu, Suh-Ching Yang, Mei Chich Hsu, Jen-Fang Liu

研究成果: 雜誌貢獻文章同行評審

8 引文 斯高帕斯(Scopus)

摘要

Background: Angelica Sinensis (AS), a folk medicine, has long been used in ergogenic aids for athletes, but there is little scientific evidence supporting its effects. We investigated whether AS induces hypertrophy in myotubes through the phosphatidylinositol 3-kinase (PI3K)/Akt (also termed PKB)/mammalian target of the rapamycin (mTOR) pathway. Methods: An in vitro experiment investigating the induction of hypertrophy in myotubes was conducted. To investigate whether AS promoted the hypertrophy of myotubes, an established in vitro model of myotube hypertrophy with and without AS was used and examined using microscopic images. The role of the PI3K/Akt/mTOR signaling pathway in AS-induced myotube hypertrophy was evaluated. Two inhibitors, wortmannin (an inhibitor of PI3K) and rapamycin (an inhibitor of mTOR), were used. Result: The results revealed that the myotube diameters in the AS-treated group were significantly larger than those in the untreated control group (P < 0.05). Wortmannin and rapamycin inhibited AS-induced hypertrophy. Furthermore, AS increased Akt and mTOR phosphorylation through the PI3K pathway and induced myotube hypertrophy. Conclusion: The results confirmed that AS induces hypertrophy in myotubes through the PI3K/Akt/mTOR pathway.
原文英語
文章編號144
期刊BMC Complementary and Alternative Medicine
14
發行號1
DOIs
出版狀態已發佈 - 五月 3 2014

ASJC Scopus subject areas

  • Complementary and alternative medicine

指紋 深入研究「Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway」主題。共同形成了獨特的指紋。

引用此