Analytical measurement of pegylated molecules

Tian Lu Cheng, Kuo Hsiang Chuang, Bing Mae Chen, Steve R. Roffler

研究成果: 雜誌貢獻回顧型文獻同行評審

87 引文 斯高帕斯(Scopus)

摘要

Attachment of poly(ethylene glycol) (PEG) to proteins, peptides, liposomes, drugs, and nanoparticles can improve pharmaceutical pharmacokinetic properties and enhance in vivo biological efficacy. Since the first PEGylated product was approved by the Food and Drug Administration in 1990, increasing numbers of PEGylated compounds have entered clinical use. Successful clinical development of PEGylated pharmaceuticals requires accurate methods for the qualitative and quantitative analysis of intact PEG conjugates in biological fluids. In this article, we review assay methods that can be utilized for the detection and measurement of PEGylated pharmaceuticals in complex biological samples for determination of biodistribution and pharmacokinetic properties. In particular, we describe relevant colorimetric, chromatographic, radiolabeled, biological, and enzyme-linked immunosorbent assays for the pharmacokinetic study of PEGylated molecules.

原文英語
頁(從 - 到)881-899
頁數19
期刊Bioconjugate Chemistry
23
發行號5
DOIs
出版狀態已發佈 - 5月 16 2012

ASJC Scopus subject areas

  • 生物技術
  • 生物工程
  • 生物醫學工程
  • 藥理
  • 藥學科學
  • 有機化學

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