Analysis of aberrant transcription of TSG101 in hepatocellular carcinomas

Y. J. Chen, P. H. Chen, S. Y. Lin, J. G. Chang

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3 引文 斯高帕斯(Scopus)

摘要

A variety of studies suggest that tumour suppressor loci on chromosome 11p are important in various forms of human neoplasia. Recently, a gene located at the chromosome 11p 15.1-15.2 region called TSG101 was discovered and proposed as a candidate tumour suppressor gene in breast cancers. We evaluated the TSG101 gene in a panel of liver cancer cell lines and paired tumours and non-malignant tissues. In this study, four of the seven (57%) cell lines, eight of the 18 (44%) tumours and four of the 18 (22%) non- malignant liver tissues exhibited aberrant TSG101 transcripts by nested reverse transcription-polymerase chain reaction (RT-PCR) analysis. However, a normal-sized transcript without sequence abnormalities verified by single- stranded conformation polymorphism (SSCP) analysis was expressed at robust levels in all the cell lines and most of the tissue samples tested. In addition, Southern blot analysis could identify no genomic abnormalities of the gene. Our results suggest either that the TSG101 gene may not be involved in hepatocarcinogenesis or that it plays a role in the development and/or progress of hepatocellular carcinomas through an unusual mechanism.

原文英語
頁(從 - 到)302-308
頁數7
期刊European Journal of Cancer
35
發行號2
DOIs
出版狀態已發佈 - 二月 1999

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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