An on-spot internal standard addition approach for accurately determining colistin A and colistin B in dried blood spots using ultra high-performance liquid chromatography–tandem mass spectrometry

I. Lin Tsai, Ching Hua Kuo, Hsin Yun Sun, Yu Chung Chuang, Divyabharathi Chepyala, Shu Wen Lin, Yun Jung Tsai

研究成果: 雜誌貢獻文章

2 引文 (Scopus)

摘要

Outbreaks of multidrug-resistant Gram-negative bacterial infections have been reported worldwide. Colistin, an antibiotic with known nephrotoxicity and neurotoxicity, is now being used to treat multidrug-resistant Gram-negative strains. In this study, we applied an on-spot internal standard addition approach coupled with an ultra high-performance liquid chromatography-tandem mass spectrometry (LC–MS/MS) method to quantify colistin A and B from dried blood spots (DBSs). Only 15 μL of whole blood was required for each sample. An internal standard with the same yield of extraction recoveries as colistin was added to the spot before sample extraction for accurate quantification. Formic acid in water (0.15%) with an equal volume of acetonitrile (50:50 v/v) was used as the extraction solution. With the optimized extraction process and LC–MS/MS conditions, colistin A and B could be quantified from a DBS with respective limits of quantification of 0.13 and 0.27 μg mL−1, and the retention times were < 2 min. The relative standard deviations of within-run and between-run precisions for peak area ratios were all < 17.3%. Accuracies were 91.5-111.2% for lower limit of quantification, low, medium, and high QC samples. The stability of the easily hydrolyzed prodrug, colistin methanesulfonate, was investigated in DBSs. Less than 4% of the prodrug was found to be hydrolyzed in DBSs at room temperature after 48 h. The developed method applied an on-spot internal standard addition approach which benefited the precision and accuracy. Results showed that DBS sampling coupled with the sensitive LC–MS/MS method has the potential to be an alternative approach for colistin quantification, where the bias of prodrug hydrolysis in liquid samples is decreased.

原文英語
頁(從 - 到)783-793
頁數11
期刊Journal of Pharmaceutical and Biomedical Analysis
145
DOIs
出版狀態已發佈 - 十月 25 2017

指紋

Colistin
Mass spectrometry
Mass Spectrometry
Blood
Prodrugs
Liquids
formic acid
Gram-Negative Bacterial Infections
High performance liquid chromatography
Tandem Mass Spectrometry
Disease Outbreaks
Hydrolysis
High Pressure Liquid Chromatography
Sampling
Anti-Bacterial Agents
Recovery
Temperature
Water

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

引用此文

An on-spot internal standard addition approach for accurately determining colistin A and colistin B in dried blood spots using ultra high-performance liquid chromatography–tandem mass spectrometry. / Tsai, I. Lin; Kuo, Ching Hua; Sun, Hsin Yun; Chuang, Yu Chung; Chepyala, Divyabharathi; Lin, Shu Wen; Tsai, Yun Jung.

於: Journal of Pharmaceutical and Biomedical Analysis, 卷 145, 25.10.2017, p. 783-793.

研究成果: 雜誌貢獻文章

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abstract = "Outbreaks of multidrug-resistant Gram-negative bacterial infections have been reported worldwide. Colistin, an antibiotic with known nephrotoxicity and neurotoxicity, is now being used to treat multidrug-resistant Gram-negative strains. In this study, we applied an on-spot internal standard addition approach coupled with an ultra high-performance liquid chromatography-tandem mass spectrometry (LC–MS/MS) method to quantify colistin A and B from dried blood spots (DBSs). Only 15 μL of whole blood was required for each sample. An internal standard with the same yield of extraction recoveries as colistin was added to the spot before sample extraction for accurate quantification. Formic acid in water (0.15{\%}) with an equal volume of acetonitrile (50:50 v/v) was used as the extraction solution. With the optimized extraction process and LC–MS/MS conditions, colistin A and B could be quantified from a DBS with respective limits of quantification of 0.13 and 0.27 μg mL−1, and the retention times were < 2 min. The relative standard deviations of within-run and between-run precisions for peak area ratios were all < 17.3{\%}. Accuracies were 91.5-111.2{\%} for lower limit of quantification, low, medium, and high QC samples. The stability of the easily hydrolyzed prodrug, colistin methanesulfonate, was investigated in DBSs. Less than 4{\%} of the prodrug was found to be hydrolyzed in DBSs at room temperature after 48 h. The developed method applied an on-spot internal standard addition approach which benefited the precision and accuracy. Results showed that DBS sampling coupled with the sensitive LC–MS/MS method has the potential to be an alternative approach for colistin quantification, where the bias of prodrug hydrolysis in liquid samples is decreased.",
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AU - Tsai, I. Lin

AU - Kuo, Ching Hua

AU - Sun, Hsin Yun

AU - Chuang, Yu Chung

AU - Chepyala, Divyabharathi

AU - Lin, Shu Wen

AU - Tsai, Yun Jung

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N2 - Outbreaks of multidrug-resistant Gram-negative bacterial infections have been reported worldwide. Colistin, an antibiotic with known nephrotoxicity and neurotoxicity, is now being used to treat multidrug-resistant Gram-negative strains. In this study, we applied an on-spot internal standard addition approach coupled with an ultra high-performance liquid chromatography-tandem mass spectrometry (LC–MS/MS) method to quantify colistin A and B from dried blood spots (DBSs). Only 15 μL of whole blood was required for each sample. An internal standard with the same yield of extraction recoveries as colistin was added to the spot before sample extraction for accurate quantification. Formic acid in water (0.15%) with an equal volume of acetonitrile (50:50 v/v) was used as the extraction solution. With the optimized extraction process and LC–MS/MS conditions, colistin A and B could be quantified from a DBS with respective limits of quantification of 0.13 and 0.27 μg mL−1, and the retention times were < 2 min. The relative standard deviations of within-run and between-run precisions for peak area ratios were all < 17.3%. Accuracies were 91.5-111.2% for lower limit of quantification, low, medium, and high QC samples. The stability of the easily hydrolyzed prodrug, colistin methanesulfonate, was investigated in DBSs. Less than 4% of the prodrug was found to be hydrolyzed in DBSs at room temperature after 48 h. The developed method applied an on-spot internal standard addition approach which benefited the precision and accuracy. Results showed that DBS sampling coupled with the sensitive LC–MS/MS method has the potential to be an alternative approach for colistin quantification, where the bias of prodrug hydrolysis in liquid samples is decreased.

AB - Outbreaks of multidrug-resistant Gram-negative bacterial infections have been reported worldwide. Colistin, an antibiotic with known nephrotoxicity and neurotoxicity, is now being used to treat multidrug-resistant Gram-negative strains. In this study, we applied an on-spot internal standard addition approach coupled with an ultra high-performance liquid chromatography-tandem mass spectrometry (LC–MS/MS) method to quantify colistin A and B from dried blood spots (DBSs). Only 15 μL of whole blood was required for each sample. An internal standard with the same yield of extraction recoveries as colistin was added to the spot before sample extraction for accurate quantification. Formic acid in water (0.15%) with an equal volume of acetonitrile (50:50 v/v) was used as the extraction solution. With the optimized extraction process and LC–MS/MS conditions, colistin A and B could be quantified from a DBS with respective limits of quantification of 0.13 and 0.27 μg mL−1, and the retention times were < 2 min. The relative standard deviations of within-run and between-run precisions for peak area ratios were all < 17.3%. Accuracies were 91.5-111.2% for lower limit of quantification, low, medium, and high QC samples. The stability of the easily hydrolyzed prodrug, colistin methanesulfonate, was investigated in DBSs. Less than 4% of the prodrug was found to be hydrolyzed in DBSs at room temperature after 48 h. The developed method applied an on-spot internal standard addition approach which benefited the precision and accuracy. Results showed that DBS sampling coupled with the sensitive LC–MS/MS method has the potential to be an alternative approach for colistin quantification, where the bias of prodrug hydrolysis in liquid samples is decreased.

KW - Colistin

KW - Dried blood spot

KW - Mass spectrometry

KW - On-spot internal standard addition

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