An intrinsically disordered protein interacts with the cytoskeleton for adaptive root growth under stress

An Shan Hsiao, Kuan Wang, Tuan Hua David Ho

研究成果: 雜誌貢獻文章同行評審

1 引文 斯高帕斯(Scopus)

摘要

Intrinsically disordered proteins function as flexible stress modulators in vivo through largely unknown mechanisms. Here, we elucidated the mechanistic role of an intrinsically disordered protein, REPETITIVE PRO-RICH PROTEIN (RePRP), in regulating rice (Oryza sativa) root growth under water deficit. With nearly 40% Pro, RePRP is induced by water deficit and abscisic acid (ABA) in the root elongation zone. RePRP is sufficient and necessary for repression of root development by water deficit or ABA. We showed that RePRP interacts with the highly ordered cytoskeleton components actin and tubulin both in vivo and in vitro. Binding of RePRP reduces the abundance of actin filaments, thus diminishing noncellulosic polysaccharide transport to the cell wall and increasing the enzyme activity of Suc synthase. RePRP also reorients the microtubule network, which leads to disordered cellulose microfibril organization in the cell wall. The cell wall modification suppresses root cell elongation, thereby generating short roots, whereas increased Suc synthase activity triggers starch accumulation in "heavy"roots. Intrinsically disordered proteins control cell elongation and carbon reserves via an order-by-disorder mechanism, regulating the highly ordered cytoskeleton for development of "short-but-heavy"roots as an adaptive response to water deficit in rice.
原文英語
頁(從 - 到)570-587
頁數18
期刊Plant Physiology
183
發行號2
DOIs
出版狀態已發佈 - 6月 2020

ASJC Scopus subject areas

  • 生理學
  • 遺傳學
  • 植物科學

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