An experimentally validated genome-scale metabolic reconstruction of Klebsiella pneumoniae MGH 78578, iYL1228

Yu Chieh Liao, Tzu Wen Huang, Feng Chi Chen, Pep Charusanti, Jay S.J. Hong, Hwan You Chang, Shih Feng Tsai, Bernhard O. Palsson, Chao A. Hsiung

研究成果: 雜誌貢獻文章同行評審

77 引文 斯高帕斯(Scopus)

摘要

Klebsiella pneumoniae is a Gram-negative bacterium of the family Enterobacteriaceae that possesses diverse metabolic capabilities: many strains are leading causes of hospital-acquired infections that are often refractory to multiple antibiotics, yet other strains are metabolically engineered and used for production of commercially valuable chemicals. To study its metabolism, we constructed a genome-scale metabolic model (iYL1228) for strain MGH 78578, experimentally determined its biomass composition, experimentally determined its ability to grow on a broad range of carbon, nitrogen, phosphorus and sulfur sources, and assessed the ability of the model to accurately simulate growth versus no growth on these substrates. The model contains 1,228 genes encoding 1,188 enzymes that catalyze 1,970 reactions and accurately simulates growth on 84% of the substrates tested. Furthermore, quantitative comparison of growth rates between the model and experimental data for nine of the substrates also showed good agreement. The genome-scale metabolic reconstruction for K. pneumoniae presented here thus provides an experimentally validated in silico platform for further studies of this important industrial and biomedical organism.

原文英語
頁(從 - 到)1710-1717
頁數8
期刊Journal of Bacteriology
193
發行號7
DOIs
出版狀態已發佈 - 四月 2011
對外發佈

ASJC Scopus subject areas

  • 微生物學
  • 分子生物學

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