An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration

V. Chia Hsuan Tseng, Chee Ho Chew, Wan Ting Huang, Yang Kao Wang, Ko Shao Chen, Szu Yi Chou, Chien Chung Chen

研究成果: 雜誌貢獻文章同行評審

7 引文 斯高帕斯(Scopus)

摘要

Recently, a novel substrate known as an electrospun polylactic acid (PLLA) microtube array membrane (MTAM) was successfully developed as a cell coculture platform. Structurally, this substrate is made up of one-to-one connected, ultrathin, submicron scale fibers that are arranged in an arrayed formation. Its unique structure confers several key advantages which are beneficial in a cell coculture system. In this study, the interaction between rat fetal neural stem cells (NSC) and astrocytes was examined by comparing the outcome of a typical Transwell-based coculture system and that of an electrospun PLLA MTAM-based coculture system. Compared to tissue culture polystyrene (TCP) and Transwell coculture inserts, a superior cell viability of NSC was observed when cultured in lumens of electrospun PLLA MTAM (with supportive immunostaining images). Reverse transcription polymerase chain reaction revealed a strong interaction between astrocytes and NSC through a higher expression of doublecortin and a lower expression of nestin. These data demonstrate that MTAM is clearly a better coculture platform than the traditional Transwell system.
原文英語
頁(從 - 到)179-190
頁數12
期刊Cells Tissues Organs
204
發行號3-4
DOIs
出版狀態已發佈 - 十月 1 2017

ASJC Scopus subject areas

  • 解剖學
  • 組織學

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