An apoptosis-related gene network induced by novel compound-cRGD in human breast cancer cells

Tsui Chin Huang, Hsuan Cheng Huang, Chih Chin Chang, Hsin Yi Chang, Chern Han Ou, Chun Hua Hsu, Shui Tein Chen, Hsueh Fen Juan

研究成果: 雜誌貢獻文章

10 引文 (Scopus)

摘要

Synthetic peptides with the arginine-glycine-aspartate (RGD) motif have been used widely as inhibitors of integrin-ligand interactions to study cell growth, adhesion, migration and differentiation. We designed cyclic-RGD peptide (Tpa-RGDWPC-, cRGD) which could cause cell death in MCF-7 cell line. In order to understand the mechanism involved in cRGD-induced apoptosis, we used microarray, real-time quantitative PCR (Q-PCR) and bioinformatics to study the effects of cRGD on breast cancer cell line MCF-7. By time-series gene expression measurements and our developed software BSIP and GeneNetwork, we reconstructed an apoptosis-related gene network. In the network, caspase-9, located at the upstream, activates downstream effector caspases such as caspase-7, leading to the induction of caspase-4. Combined our previous proteomics data with newly performed docking simulation, it indicated that the cell death induced by cRGD may be triggered through blocking integrin signaling to the extracellular matrix and activation of caspase pathway. This study provides a molecular explanation of cRGD treatment in breast cancer cells and set forth a constructive far-reaching impact on breast cancer therapy.
原文英語
頁(從 - 到)3517-3522
頁數6
期刊FEBS Letters
581
發行號18
DOIs
出版狀態已發佈 - 七月 24 2007

指紋

Gene Regulatory Networks
Genes
Cells
Cell death
Apoptosis
Breast Neoplasms
Caspases
Integrins
Cell Death
Effector Caspases
Caspase 7
Cell Line
Cyclic Peptides
Caspase 9
MCF-7 Cells
Cell growth
Bioinformatics
Microarrays
Computational Biology
Gene expression

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

引用此文

An apoptosis-related gene network induced by novel compound-cRGD in human breast cancer cells. / Huang, Tsui Chin; Huang, Hsuan Cheng; Chang, Chih Chin; Chang, Hsin Yi; Ou, Chern Han; Hsu, Chun Hua; Chen, Shui Tein; Juan, Hsueh Fen.

於: FEBS Letters, 卷 581, 編號 18, 24.07.2007, p. 3517-3522.

研究成果: 雜誌貢獻文章

Huang, Tsui Chin ; Huang, Hsuan Cheng ; Chang, Chih Chin ; Chang, Hsin Yi ; Ou, Chern Han ; Hsu, Chun Hua ; Chen, Shui Tein ; Juan, Hsueh Fen. / An apoptosis-related gene network induced by novel compound-cRGD in human breast cancer cells. 於: FEBS Letters. 2007 ; 卷 581, 編號 18. 頁 3517-3522.
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AU - Ou, Chern Han

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AU - Chen, Shui Tein

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