TY - JOUR
T1 - An actinobacterial isolate, streptomyces sp. Yx44, produces broad-spectrum antibiotics that strongly inhibit staphylococcus aureus
AU - Chang, Tien Lin
AU - Huang, Tzu Wen
AU - Wang, Ying Xuan
AU - Liu, Chang Pan
AU - Kirby, Ralph
AU - Chu, Chien Ming
AU - Huang, Chih Hung
N1 - Funding Information:
We thank Mackay Memorial Hospital for testing the inhibition ability of YX44 fermentation broth against their clinical collection of multidrug-resistant S. aureus strains. We also thank the Proteomics Research Center of National Yang-Ming University for their help with the GFC filtration and ESI-MS analysis.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/3
Y1 - 2021/3
N2 - The need for new antibiotics is increasing due to their overuse, and antibiotic resistance has become one of the major threats worldwide to public health, food safety, and clinical treatment. In this study, we describe an actinobacterial isolate, YX44, which belongs to the genus Streptomyces. This Streptomyces was isolated from a drinking pipe located in Osaka, Japan, and has the ability to inhibit Gram-positive bacteria, Gram-negative bacteria, and various fungi. YX44 fermentation broth shows strong activity against Escherichia coli and Staphylococcus aureus, as well as also inhibiting clinical isolates of multidrug-resistant Staphylococcus aureus. The YX44 antibacterial substances in the broth are relatively heat-stable, show high stability from the pH range 1 to 11, and have good solubility in both organic and non-organic solvents. Size-exclusion chromatography revealed that the YX44 antibacterial compounds are less than 1000 Da in size. LC-MS was able to identify three possible candidate molecules with molecular weights of 308, 365, 460, and 653 g/mol; none of these sizes correspond to any well-known antibiotics. Our results show that Streptomyces sp. YX44 seems to produce a number of novel antibiotics with high pH stability and good solubility that have significant activity against S. aureus, including multidrug-resistant strains.
AB - The need for new antibiotics is increasing due to their overuse, and antibiotic resistance has become one of the major threats worldwide to public health, food safety, and clinical treatment. In this study, we describe an actinobacterial isolate, YX44, which belongs to the genus Streptomyces. This Streptomyces was isolated from a drinking pipe located in Osaka, Japan, and has the ability to inhibit Gram-positive bacteria, Gram-negative bacteria, and various fungi. YX44 fermentation broth shows strong activity against Escherichia coli and Staphylococcus aureus, as well as also inhibiting clinical isolates of multidrug-resistant Staphylococcus aureus. The YX44 antibacterial substances in the broth are relatively heat-stable, show high stability from the pH range 1 to 11, and have good solubility in both organic and non-organic solvents. Size-exclusion chromatography revealed that the YX44 antibacterial compounds are less than 1000 Da in size. LC-MS was able to identify three possible candidate molecules with molecular weights of 308, 365, 460, and 653 g/mol; none of these sizes correspond to any well-known antibiotics. Our results show that Streptomyces sp. YX44 seems to produce a number of novel antibiotics with high pH stability and good solubility that have significant activity against S. aureus, including multidrug-resistant strains.
KW - Antibiotic
KW - Hydrophilic
KW - Multidrug-resistant Staphylococcus aureus
KW - PH stability
KW - Streptomyces
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U2 - 10.3390/microorganisms9030630
DO - 10.3390/microorganisms9030630
M3 - Article
AN - SCOPUS:85102615858
VL - 9
SP - 1
EP - 15
JO - Microorganisms
JF - Microorganisms
SN - 2076-2607
IS - 3
M1 - 630
ER -