Amphetamine activates connexin43 gene expression in cultured neonatal rat cardiomyocytes through JNK and AP-1 pathway

Kou Gi Shyu, Bao Wei Wang, Yu Hui Yang, Shiow Chwen Tsai, Shankung Lin, Chun Chung Lee

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31 引文 斯高帕斯(Scopus)

摘要

Objective: Amphetamine has been known to induce cardiac dysrhythmia and sudden death. However, the molecular mechanism for the induction of dysrhythmia is not known. Connexin43 (Cx43) plays an important role for arrhythmogenesis. This study was undertaken to test the hypothesis that amphetamine could induce Cx43 expression in cardiac myocytes. Methods: Neonatal Wistar rat cardiac myocytes were cultured under the stimulation of amphetamine. Cx43 mRNA and protein expression were examined by Northern and Western blots, respectively. We used c-Jun N-terminal kinase (JNK) inhibitor, SP600125, and JNK1 dsRNAi to investigate the signal pathway of amphetamine-induced expression of Cx43. Results: The level of Cx43 protein significantly increased from 4 to 24 h after addition of amphetamine (10 μM). The Cx43 mRNA increased maximally to 4.2-fold at 6 h after addition of amphetamine and returned to the baseline level at 48 h. These increases of Cx43 protein at 24 h were completely attenuated (P
原文英語
頁(從 - 到)98-108
頁數11
期刊Cardiovascular Research
63
發行號1
DOIs
出版狀態已發佈 - 7月 1 2004

ASJC Scopus subject areas

  • 心臟病學與心血管醫學

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