Aminoazo dye-protein-adduct enhances inhibitory effect on digestibility and damages to Gastro-Duodenal-Hepatic axis

Li Yun Lin, Chiung Chi Peng, Yeh Chen, Boa Chan Huang, Chun Chao Chang, Robert Y. Peng

研究成果: 雜誌貢獻文章


4-Dimethylaminoazobenzene (DAB, methyl yellow, or butter yellow), a human carcinogen, has been banned for use in foods since 1988. In 2014, DAB adulteration in Tofu occurred in Taiwan. We hypothesize that DAB can form [DAB·SBP]adduct adductwith soybean protein (SBP) which could damage Gastro-Duodenal-Hepatic axis. Sprague-Dawley rats gavage fed [DAB·SBP]adduct adductrevealed severely reduced body weight and damaged duodenum, liver, hepatic mitochondria, and spleen. Hepatic levels of glutathione and ATP were severely reduced. Serum GOT and GPT were substantially elevated. Analysis by the adsorption isotherm clearly revealed DAB formed very stable [DAB·SBP]adduct adductat 1:1 molar ration (Phase A). The equilibrium constant of this colloidal adduct[DAB·SBP]adductwas KeqA=α, behaving as gross]conjugate, with KeqC = 3.23×1-2 mg/mL, implicating a moderately strong adsorption. The in vitro pepsin digestibility test showed apparently reduced digestibility by 27% (by Ninhydrin assay) or 8% (by Bradford assay). Conclusively, this is the first report indicating that [DAB·SBP]adductpotentially is capable to damage the Gastro-Duodenal-Hepatic axis.

期刊PLoS One
出版狀態已發佈 - 四月 1 2017


ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)